20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.
20 years of crystal hits: progress and promise in ultrahigh-throughput crystallization screening.
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DOI：
10.1107/s2059798323001274
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发表时间：
2023-03-01
期刊：
影响因子：
--
通讯作者：
中科院分区：
文献类型：
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作者：
Hauptman-Woodward医学研究所的结晶设施，现在是国家高通量结晶中心，已经为结构生物学社区服务了二十多年。从我们的高通量结晶服务中吸取的经验教训，当前的操作和能力，以及结晶的未来愿景。基于衍射的结构方法贡献了很大一部分的生物分子结构模型，提供了一个关键的理解大分子结构。这些方法需要靶分子的结晶，这仍然是基于晶体的结构测定的主要瓶颈。Hauptman-Woodward医学研究所的国家高通量结晶中心专注于通过机器人支持的高通量筛选和先进的成像相结合来克服结晶障碍，以提高找到结晶条件的成功率。本文将介绍我们高通量结晶服务20多年的运营经验。目前的实验管道，仪器，成像能力和软件的图像查看和晶体评分进行了详细说明。反映了该领域的新发展和进一步改进生物分子结晶的机会。
The crystallization facility at Hauptman–Woodward Medical Research Institute, now the National High-Throughput Crystallization Center, has served the structural biology community for over two decades. The lessons learned from our high-throughput crystallization services, current operations and capabilities are described, together with a vision for the future of crystallization. Diffraction-based structural methods contribute a large fraction of the biomolecular structural models available, providing a critical understanding of macromolecular architecture. These methods require crystallization of the target molecule, which remains a primary bottleneck in crystal-based structure determination. The National High-Throughput Crystallization Center at Hauptman–Woodward Medical Research Institute has focused on overcoming obstacles to crystallization through a combination of robotics-enabled high-throughput screening and advanced imaging to increase the success of finding crystallization conditions. This paper will describe the lessons learned from over 20 years of operation of our high-throughput crystallization services. The current experimental pipelines, instrumentation, imaging capabilities and software for image viewing and crystal scoring are detailed. New developments in the field and opportunities for further improvements in biomolecular crystallization are reflected on.
影响因子：
3.7
作者：
Fusco D;Barnum TJ;Bruno AE;Luft JR;Snell EH;Mukherjee S;Charbonneau P
通讯作者：
Charbonneau P