High-frequency electron paramagnetic resonance of metal-containing porphyrin compounds using a microcantilever
High-frequency electron paramagnetic resonance of metal-containing porphyrin compounds using a microcantilever
复制标题
使用微悬臂梁对含金属卟啉化合物进行高频电子顺磁共振
DOI：
10.1016/j.jinorgbio.2016.04.016
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发表时间：
2016
影响因子：
3.9
通讯作者：
Hitoshi Ohta
中科院分区：
文献类型：
--
作者：
Eiji Ohmichi;Tsubasa Okamoto;Masaaki Mitani;Hideyuki Takahashi;Hitoshi Ohta
在本文中，我们报道了一种利用微悬臂梁进行高频电子顺磁共振(HFEPR)的新技术。在这种方法中，样品被安装在悬臂梁上，与EPR吸收相关的场梯度力被检测为悬臂梁弯曲。通过使用微米尺寸的悬臂梁，该技术可以应用于μg量级的非常微小的样品。此外，压阻悬臂梁的使用使实验装置变得简单和紧凑。在这项研究中，我们将这项技术应用于含金属卟啉化合物的多频率HFEPR测量，这些化合物是含金属蛋白质和辅酶(如血红蛋白和氰基钴胺)的重要组成元素。
In this article, we report a novel technique of high-frequency electron paramagnetic resonance (HFEPR) using a microcantilever. In this method, a sample is mounted on a cantilever, and the field-gradient force associated with EPR absorption is detected as a cantilever bending. By using a micrometer-sized cantilever, this technique can be applied to a very tiny sample on the order ofμg. In addition, the use of a piezoresistive cantilever makes the experimental setup easy and compact. In this study, we applied this technique to multi-frequency HFEPR measurements of metal-containing porphyrin compounds, which are an important composing element of metal-containing proteins and coenzymes such as hemoglobin and cyanocobalamin.