Regulation of the expression of the angiogenic enzyme platelet-derived endothelial cell growth factor/thymidine phosphorylase in endometrial isolates by ovarian steroids and cytokines.
Regulation of the expression of the angiogenic enzyme platelet-derived endothelial cell growth factor/thymidine phosphorylase in endometrial isolates by ovarian steroids and cytokines.
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卵巢类固醇和细胞因子对子宫内膜分离物中血管生成酶血小板衍生内皮细胞生长因子/胸苷磷酸化酶表达的调节。
DOI：
10.1210/en.138.11.4921
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发表时间：
1997
期刊：
影响因子：
4.8
通讯作者：
R. Bicknell
中科院分区：
文献类型：
--
作者：
Lyna Zhang;I. Mackenzie;M. Rees;R. Bicknell
血管生成酶血小板衍生内皮细胞生长因子/胸苷磷酸化酶（PD-ECGF/TP）在黄体期和经期的子宫内膜腺中强烈表达，而在月经周期的增殖期则不表达。在间质中则相反，在增生期表达强烈，而在黄体期或经期则不然。炎症因子诱导人正常子宫内膜上皮细胞和正常子宫内膜间质细胞PD-ECGF/TP表达细胞因子对PD-ECGF/TP的诱导具有细胞依赖性。因此，在NEE细胞中，PD-ECGF/TP的表达被肿瘤坏死因子-a和干扰素- γ联合强烈诱导。相比之下，在正常子宫内膜基质细胞中，干扰素- γ迄今为止对PD-ECGF/TP的诱导作用最强。该酶的表达不受卵巢激素的单独调节。虽然单独用生理浓度的黄体酮（5 × 10[-8] M）或转化生长因子- β 1 （10 ng/ml）处理NEE细胞对PD-ECGF/TP表达没有影响，但当以相同剂量同时给药时，它们诱导表达增加48倍。这种表达与子宫内孕酮和转化生长因子- β 1水平的周期性变化有关。
The angiogenic enzyme platelet-derived endothelial cell growth factor/thymidine phosphorylase (PD-ECGF/TP) was strongly expressed in the endometrial glands in the luteal and menstrual, but not the proliferative, phases of the cycle. The converse was seen in the stroma, where expression was strong in the proliferative, but not the luteal or menstrual, phases. Inflammatory cytokines induced PD-ECGF/TP expression in primary cultures of human normal endometrial epithelial (NEE) and normal endometrial stromal cells. The profile of cytokine induction of PD-ECGF/TP was cell dependent. Thus, in NEE cells, PD-ECGF/TP expression was strongly induced by the combination tumor necrosis factor-a and interferon-gamma. In contrast, in normal endometrial stromal cells, interferon-gamma gave, by far, the strongest induction of PD-ECGF/TP. Expression of the enzyme was not regulated by ovarian hormones alone. Although treatment of NEE cells with a physiological concentration of progesterone (5 X 10[-8] M) or transforming growth factor-beta1 (10 ng/ml) alone had no effect on PD-ECGF/TP expression, when delivered together at the same dose they induced a 48-fold increase in expression. This expression correlates with cyclic changes in progesterone and transforming growth factor-beta1 levels in the uterus.
DOI：
10.1073/pnas.92.16.7362
发表时间：
1995-08-01
影响因子：
11.1
作者：
BRUNER, KL;RODGERS, WH;OSTEEN, KG
通讯作者：
OSTEEN, KG
干扰素-γ诱导肿瘤坏死因子受体的合成。
DOI：
--
发表时间：
1986
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Ruggiero,V;Tavernier,J;Fiers,W;Baglioni,C
通讯作者：
Baglioni,C