Hybridization Experiments with Deoxyribonucleic Acid from Ureaplasma urealyticum Serovars I to VIII

Hybridization Experiments with Deoxyribonucleic Acid from Ureaplasma urealyticum Serovars I to VIII
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解脲支原体血清型 I 至 VIII 脱氧核糖核酸的杂交实验

DOI:
10.1099/00207713-31-3-259
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发表时间:
1981
影响因子:
2.8
通讯作者:
E. Freundt
E. Freundt
中科院分区:
生物学3区
文献类型:
--
作者:
C. Christiansen;F. Black;E. Freundt

文献摘要

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用解脲脲原体血清型(血清变种)I至VIII的脱氧核糖核酸进行的核酸杂交实验显示,所分析的菌株聚类为两个主要群组。一个群组包括血清型I、III和VI,另一个包括血清型II、IV、V、VII和VIII,这两个群组在40% - 60%的同源性水平上有所区分。在此呈现的核酸同源性数据与已知存在于解脲脲原体不同血清型之间的异源血清学关系之间未显示出一致的相关性。另一方面,根据脱氧核糖核酸同源性的聚类与最近通过聚丙烯酰胺凝胶电泳方法所发表的观察结果之间具有一致性。
Nucleic acid hybridization experiments performed with deoxyribonucleic acid from serovars (serotypes) I to VIII of Ureaplasma urealyticum revealed a clustering of the analyzed strains into two major groups. One cluster included serovars I, III, and VI and the other included serovars II, IV, V, VII, and VIII, the two groups being distinguished at the 40 to 60% homology level. No consistent correlation could be demonstrated between the nucleic acid homology data presented here and the heterologous serological relationships known to exist between different serovars of U. urealyticum. On the other hand, agreement was found between clusterings according to deoxyribonucleic acid homologies and recently published observations made by polyacrylamide gel electrophoresis methods.