An immunocytochemist's view of gonadotropin storage in the adult male rat: cytochemical and morphological heterogeneity in serially sectioned gonadotropes.
An immunocytochemist's view of gonadotropin storage in the adult male rat: cytochemical and morphological heterogeneity in serially sectioned gonadotropes.
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免疫细胞化学家对成年雄性大鼠促性腺激素储存的看法：连续切片促性腺激素的细胞化学和形态异质性。
DOI：
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发表时间：
1980
影响因子：
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通讯作者：
L. L. Garner
中科院分区：
文献类型：
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作者：
G. Childs;D. Ellison;L. L. Garner
本研究旨在利用超微结构免疫细胞化学对一系列超薄切片阐明促性腺激素的激素储存模式。取7只正常成年雄性大鼠共6组连续切片，分别检测LH、FSH和ACTH的β链和c -末端序列，并取430个正常成年雄性大鼠的三组或双组连续切片。大约50- 80%的细胞含有LH和FSH，其中大多数是I型细胞，其特征是其圆形和分泌颗粒的异质性。仅含有卵泡刺激素或黄体生成素的细胞平均占总数的19%。从形态学上看，这是一个混合组，包括II型细胞，其特征是它们的角状形状和分泌颗粒的数量，平均直径250 nm。在FSH细胞（以及两只大鼠的少量LH细胞）中也有III型细胞，类似于促肾上腺皮质激素。平均10%的连续切片细胞仅含有ACTH。我们的研究结果表明，促性腺激素亚群的存在只含有其中一种激素，其数量足以支持这样的假设，即它们可能部分负责促性腺激素的非平行释放。此外，FSH-LH细胞对两种激素的染色强度似乎有所不同，这表明性腺致性腺细胞是一种流体，异质细胞群，能够储存两种或仅一种激素。
This study was designed to elucidate hormone storage patterns in gonadotropes with the use of ultrastructural immunocytochemistry on serial ultrathin sections. Sets of six serial sections were strained for beta chains of LH, FSH, or the C-Terminal sequence of ACTH, and 430 cells cut in triple or double serial section were collected from a group of seven normal adult male rats. Approximately 50--80% of the cells contained both LH and FSH, and most of these were Type I cells which are distinguished by their round shape and heterogeneous populations of secretion granules. Cells containing only FSH or LH constituted, on average, 19% of the population. These were a mixed group, morphologically, and included Type II cells distinguished by their angular shape and population of secretion granules, 250 nm in average diameter. Also among the FSH cells (and a few LH cells in two of the rats) were Type III cells, which resemble the corticotrope. On average, 10% of the serially sectioned cells contained only ACTH. Our findings show the presence of subpopulations of gonadotropes containing only one of the hormones, in numbers large enough to support the hypothesis that they may be partly responsible for the nonparallel release of gonadotropins. Also, the FSH-LH cells seemed to vary in their staining intensity for the two hormones, suggesting that the gondaotropes are a fluid, heterogeneous population of cells capable of storing both or only one of the hormones.