Negative growth regulation of oncogene-transformed human breast epithelial cells by phytochemicals. Role of apoptosis.
Negative growth regulation of oncogene-transformed human breast epithelial cells by phytochemicals. Role of apoptosis.
复制标题
植物化学物质对癌基因转化的人乳腺上皮细胞的负生长调节。
DOI：
10.1111/j.1749-6632.1999.tb08742.x
复制
发表时间：
1999
影响因子：
5.2
通讯作者：
Telang,NT
中科院分区：
文献类型：
--
作者：
Katdare,M;Jinno,H;Osborne,MP;Telang,NT
Evidence from epidemiological investigations and from experiments on animal models supports the concept that natural phytochemicals present in fresh fruits, vegetables, and grain products may offer protection against human organ-site carcinogenesis. 1–5 Such phytochemicals as phytoalexins, terpenoids, flavanoids, and retinoids, either as single agents or as adjuvants, may represent valuable lead compounds for preventive/therapeutic intervention. Clinically relevant mechanistic evidence for the efficacy of phytochemicals, at present, is largely dependent on extrapolation and is therefore equivocal. Human tissue–derived in vitro models and mechanistic end point biomarkers provide an innovative approach for reducing a need for extrapolation of basic research data for their clinical relevance. Our earlier studies have sought to develop reliable models from explant and cell cultures of noncancerous breast tissue, wherein induction and modulation of chemical carcinogen-or oncogene-induced preneoplastic transformation is quantified at molecular, biochemical, and cellular levels using a spectrum of mechanistic biomarker assays. 6–11 In the multistep process of human breast carcinogenesis, transformation of target epithelial tissue from the terminal duct lobular unit (TDLU) to simple hyperplasia, atypical hyperplasia, lobular carcinoma in situ, and ductal carcinoma in situ exhibits progressively increased risk for developing aggressive breast cancer. 12 Furthermore, the comedo type of ductal carcinoma in situ that lacks estrogen receptor (ER) frequently overexpresses HER-2/neu, mutant p53, and epidermal growth factor receptor (EGFR). These lesions are at higher risk for developing drug-resistant, invasive breast cancer. 13–15 A cell culture model exhibiting these characteristics represents a valuable experimental system for clinically relevant translational research. To this end, efforts are focused to establish epithelial cell cultures from reduction mammoplasty-derived normal breast tissue, and proliferative disease without atypia, atypical aAddress for communication: Strang Cancer Research Laboratory, The Rockefeller University,
