Structure and sequence of the Drosophila melanogaster calmodulin gene.
Structure and sequence of the Drosophila melanogaster calmodulin gene.
复制标题
果蝇钙调蛋白基因的结构和序列。
DOI：
10.1016/0022-2836(87)90025-8
复制
发表时间：
1987
影响因子：
5.6
通讯作者：
Beckingham,K
中科院分区：
文献类型：
--
作者：
Smith,VL;Doyle,KE;Maune,JF;Munjaal,RP;Beckingham,K
一系列与黑腹果蝇钙调蛋白基因座重叠的噬菌体克隆已被分离，并且基因的外显子在这些克隆内定位和测序。电鳗的钙调蛋白 cDNA 克隆被用来鉴定这些克隆并定位该基因的两个主要蛋白质编码外显子。 D. cDNA 克隆然后分离、表征黑腹钙调蛋白并用于鉴定剩余的外显子。该基因由四个外显子组成，由三个长度为 3400 至 4300 个碱基的内含子分隔。外显子1由5'非翻译区和起始子ATG组成；外显子2编码氨基酸残基1至58.3；外显子3编码残基58.3至139.3；外显子4编码残基139.3至148和3'非翻译区。根据 3' 非翻译区的序列和 cDNA 克隆的长度，指示了两个或三个聚腺苷酸化位点。已经鉴定出可能参与基因转录控制和 mRNA 产物剪接的序列。 D.内含子-外显子结构的比较黑腹钙调蛋白基因、鸡钙调蛋白基因和肌钙蛋白 C 超家族的其他基因强化了先前的假设，即这些基因起源于共同的祖细胞，并允许鉴定可能存在于祖细胞基因结构中的四个内含子。 TheD.黑腹钙调蛋白基因包含其中三个内含子，而小鸡基因则包含所有四个内含子。这些基因比较还表明，这些基因编码 Ca2+ 结合环 3 的区域在结构上高度可变。小鸡和D.黑腹钙调蛋白基因在该区域有所不同，小鸡基因在此含有第五个内含子，而 D. 内含子则不存在。黑素基因。
A series of phage clones overlapping the single calmodulin gene locus ofDrosophila melanogasterhas been isolated and the exons of the gene positioned and sequenced within these clones. A calmodulin cDNA clone of the electric eel was used to identify these clones and to position the two major protein-coding exons of the gene. cDNA clones forD. melanogastercalmodulin were then isolated, characterized and used to identify the remaining exons. The gene consists of four exons separated by three introns of 3400 to 4300 bases in length. Exon 1 consists of the 5′ untranslated region and the initiator ATG; exon 2 encodes amino acid residues 1 to 58.3; exon 3 encodes residues 58.3 to 139.3; and exon 4 encodes residues 139.3 to 148 and the 3′ untranslated region. From the sequence of the 3′ untranslated region and the lengths of the cDNA clones, two or three polyadenylation sites are indicated. Sequences potentially involved in the control of transcription of the gene and splicing of the mRNA product have been identified. Comparison of the intron-exon structures of theD. melanogastercalmodulin gene, the chick calmodulin gene, and other genes of the troponin C superfamily reinforces previous hypotheses that these genes arose from a common progenitor and permits identification of four introns that were probably present in the progenitor gene structure. TheD. melanogastercalmodulin gene contains three of these introns, and the chick gene contains all four. These gene comparisons also indicate that the region of these genes encoding Ca2+-binding loop 3 is highly variable in structure. The chick andD. melanogastercalmodulin genes differ in this region, the chick gene containing a fifth intron here that is absent from theD. melanogastergene.