Three-stage enzymatic hydrolysis of steam-exploded corn stover at high substrate concentration.
Three-stage enzymatic hydrolysis of steam-exploded corn stover at high substrate concentration.
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DOI：
10.1016/j.biortech.2010.12.047
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发表时间：
2011-04
影响因子：
11.4
通讯作者：
Jing Yang;Xiaoping Zhang;Q. Yong;Shiyuan Yu
中科院分区：
文献类型：
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作者：
Jing Yang;Xiaoping Zhang;Q. Yong;Shiyuan Yu
研究了高底物浓度下汽爆玉米秸秆三段水解的可行性。当底物浓度为30%，酶负荷为15- 30 FPU/g纤维素时，采用3段（9+9+ 12 h）水解，30 h内水解率可达59.9-81.4%。与一段水解72 h相比，水解得率可提高34-37%。以汽爆玉米秸秆为底物，在底物浓度为25%，酶用量为20 FPU/g纤维素的条件下，酶解得率为85.1%，比商品纤维素酶在相同条件下的水解率高19%。结果表明，去除纤维素酶的终产物有利于纤维素酶在底物上的吸附，提高酶解产率。
The feasibility of three-stage hydrolysis of steam-exploded corn stover at high-substrate concentration was investigated. When substrate concentration was 30% and enzyme loading was 15–30FPU/g cellulose, three-stage (9+9+12h) hydrolysis could reach a hydrolysis yield of 59.9–81.4% in 30h. Compared with one-stage hydrolysis for 72h, an increase of 34–37% in hydrolysis yield could be achieved. When steam-exploded corn stover was used as the substrate for enzyme synthesis and hydrolysis was conducted at a substrate concentration of 25% with an enzyme loading of 20FPU/g cellulose, a hydrolysis yield of 85.1% was obtained, 19% higher than that the commercial cellulase could reach under the same conditions. The removal of end products was suggested to improve the adsorption of cellulase on the substrate and enhance the productivity of enzymatic hydrolysis.