Genealogy of principal strains of the yeast genetic stock center.
Genealogy of principal strains of the yeast genetic stock center.
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DOI：
10.1093/genetics/113.1.35
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发表时间：
1986-05
期刊：
影响因子：
3.3
通讯作者：
R. Mortimer;J. R. Johnston
中科院分区：
文献类型：
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作者：
R. Mortimer;J. R. Johnston
我们已经构建了一个谱系菌株S288 C，从其中许多突变体和分离株目前用于研究酿酒酵母的遗传学和分子生物学已经派生。确定了其6个祖菌株为EM 93、EM 126、NRRL YB-210和3个焙烤菌株Yeast Foam、FLD和LK。我们估计，S288 C的基因库中约88%由菌株EM 93贡献。分离株EM 93 -1C和EM 93 -3B是主要的祖先基因型，最初由C. C. Lindegren的几个实验室。我们分析了菌株EM 93的冻干培养物的分离物，并确定其基因型为MATa/MAT α SUC 2/SUC 2 GAL 2/gal 2 MAL/MAL mel/mel CUP 1/cup 1 FLO 1/flo 1。因此，菌株EM 93可能是S288 C的基因SUC 2、gal 2、CUP 1和flo 1的起源。我们给出了几个祖菌株的当前可用性的细节，并提出，这家谱应该是在阐明几种类型的遗传和分子的起源在酵母属的异质性的援助。
We have constructed a genealogy of strain S288C, from which many of the mutant and segregant strains currently used in studies on the genetics and molecular biology of Saccharomyces cerevisiae have been derived. We have determined that its six progenitor strains were EM93, EM126, NRRL YB-210 and the three baking strains Yeast Foam, FLD and LK. We have estimated that approximately 88% of the gene pool of S288C is contributed by strain EM93. The principal ancestral genotypes were those of segregant strains EM93-1C and EM93-3B, initially distributed by C. C. Lindegren to several laboratories. We have analyzed an isolate of lyophilized culture of strain EM93 and determined its genotype as MATa/MAT alpha SUC2/SUC2 GAL2/gal2 MAL/MAL mel/mel CUP1/cup1 FLO1/flo1. Strain EM93 is therefore the probable origin of genes SUC2, gal2, CUP1 and flo1 of S288C. We give details of the current availability of several of the progenitor strains and propose that this genealogy should be of assistance in elucidating the origins of several types of genetic and molecular heterogeneities in Saccharomyces.