Absorption and Metabolism of Luteolin and Its Glycosides from the Extract of Chrysanthemum morifolium Flowers in Rats and Caco-2 Cells.
Absorption and Metabolism of Luteolin and Its Glycosides from the Extract of Chrysanthemum morifolium Flowers in Rats and Caco-2 Cells.
复制标题
DOI：
10.1021/acs.jafc.5b00232
复制
发表时间：
2015-04
影响因子：
6.1
通讯作者：
Michiko Yasuda;Kotone Fujita;T. Hosoya;S. Imai;K. Shimoi
中科院分区：
文献类型：
--
作者：
Michiko Yasuda;Kotone Fujita;T. Hosoya;S. Imai;K. Shimoi
为了阐明菊花中木犀草素及其苷的生物利用度，使用 HPLC 和 LC-MS 研究了大鼠和 Caco-2 细胞中木犀草素的吸收和代谢。大鼠口服菊花提取物（1.7 g/kg体重（bw），分别相当于22.8和58.3 μmol/kg bw的木犀草素和木犀草素-7-O-葡萄糖苷）后，木犀草素及其苷很快被吸收，并在血浆中检测到木犀草素、木犀草素单葡萄糖苷和木犀草素单葡萄糖苷酸。它们的水平在给药后 1 小时达到最高（0.76 ± 0.27 μM）。在用桑叶提取物处理的 Caco-2 细胞的基底外侧的培养基中也检测到了这些化合物。这些结果表明，在施用菊花花提取物后，木犀草素和木犀草素单葡糖苷被迅速吸收，并且木犀草素、木犀草素单葡糖苷和木犀草素单葡糖苷酸可能在人体中循环。
To elucidate the bioavailability of luteolin and its glycosides in Chrysanthemum morifolium flowers, the absorption and metabolism of luteolin from them was investigated in rats and Caco-2 cells using HPLC and LC-MS. After oral administration of C. morifolium extract (1.7 g/kg body weight (bw), equivalent to 22.8 and 58.3 μmol/kg bw of luteolin and luteolin-7-O-glucoside, respectively) to rats, luteolin and its glycosides were quickly absorbed and luteolin, luteolin monoglucoside, and luteolin monoglucuronide were detected in the plasma. Their levels were highest at 1 h after administration (0.76 ± 0.27 μM). These compounds were also detected in media on the basolateral side from Caco-2 cells treated with the C. morifolium extract. These results suggest that luteolin and luteolin monoglucoside are rapidly absorbed after administration of C. morifolium flower extract and that luteolin, luteolin monoglucoside, and luteolin monoglucuronide may circulate in humans.