Improvement of CHO cell culture medium formulation:: Simultaneous substitution of glucose and glutamine
Improvement of CHO cell culture medium formulation:: Simultaneous substitution of glucose and glutamine
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DOI：
10.1021/bp990124j
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发表时间：
2000-01-01
影响因子：
2.9
通讯作者：
Gòdia, F
中科院分区：
文献类型：
--
作者：
Altamirano, C;Paredes, C;Gòdia, F
对中国仓鼠卵巢(CHO)细胞系的培养液配方进行了研究，以同时替代葡萄糖和谷氨酰胺这两种最常用的碳源和氮源，以达到更有效地利用这些化合物的目的，同时避免在培养液中积累乳酸和铵。这一过程中的关键因素是选择代谢缓慢的化合物。在所研究的不同化合物中，半乳糖和谷氨酸提供了最好的结果，允许在营养吸收和细胞需求之间实现最佳平衡的情况下支持细胞生长，并产生最少量的乳酸和铵。所获得的结果还突出了细胞对其新陈代谢的重新分配能力，以此作为对介质成分变化的反应。
The formulation of the culture medium for a Chinese hamster ovary (CHO) cell line has been investigated in terms of the simultaneous replacement of glucose and glutamine, the most commonly employed carbon and nitrogen sources, pursuing the objective of achieving a more efficient use of these compounds, simultaneously avoiding the accumulation of lactate and ammonium in the medium. The key factor in this process is the selection of compounds that are slowly metabolized. Among the different compounds studied, galactose and glutamate provide the best results, allowing support of cell growth with an optimal balance between nutrient uptake and cell requirements and the generation of minimal quantities of lactate and ammonium. The attained results also highlight the capacity of the cells to redistribute their metabolism as a response to the changes in medium composition.