Histone H2A.Z has a conserved function that is distinct from that of the major H2A sequence variants
Histone H2A.Z has a conserved function that is distinct from that of the major H2A sequence variants
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DOI：
10.1093/nar/28.19.3811
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发表时间：
2000-10-01
影响因子：
14.9
通讯作者：
Gorovsky, MA
中科院分区：
文献类型：
--
作者：
Jackson, JD;Gorovsky, MA
酿酒酵母含有编码组蛋白H2A基因家族成员的三个基因。最后一个被发现的是HTZ 1（也称为HTA 3），编码高度保守的H2A. Z类组蛋白的成员。很少有人知道它在体内的功能相比，更好的研究基因（HTA1和HTA2）编码的两个主要的H2As，我们在这里表明，虽然HTZ1基因编码H2A.Z是不是必不可少的芽殖酵母，其中断导致缓慢的生长和甲酰胺敏感性。使用质粒改组实验，我们表明主要H2A基因不能提供HTZ 1的功能，HTZ 1基因不能提供编码主要H2A的基因的基本功能，我们还首次证明了H2A.Z基因在功能上是保守的，通过显示编码纤毛原生动物嗜热四膜虫H2A.Z变体的基因能够拯救与H2A.Z相关的表型。破坏酵母HTZ1基因。因此，H2A.Z的功能不同于主要H2A的功能，并且是高度保守的。
Saccharomyces cerevisiae contains three genes that encode members of the histone H2A gene family. The last of these to be discovered, HTZ1 (also known as HTA3), encodes a member of the highly conserved H2A.Z class of histones. Little is known about how its in vivo function compares with that of the better studied genes (HTA1 and HTA2) encoding the two major H2As, We show here that, while the HTZ1 gene encoding H2A.Z is not essential in budding yeast, its disruption results in slow growth and formamide sensitivity. Using plasmid shuffle experiments, we show that the major H2A genes cannot provide the function of HTZ1 and the HTZ1 gene cannot provide the essential function of the genes encoding the major H2As, We also demonstrate for the first time that H2A.Z genes are functionally conserved by showing that the gene encoding the H2A.Z variant of the ciliated protozoan Tetrahymena thermophila is able to rescue the phenotypes associated with disruption of the yeast HTZ1 gene. Thus, the functions of H2A.Z are distinct from those of the major H2As and are highly conserved.