A Novel Vaccine Delivery Model of the Apicomplexan Eimeria tenella Expressing Eimeria maxima Antigen Protects Chickens against Infection of the Two Parasites.
A Novel Vaccine Delivery Model of the Apicomplexan Eimeria tenella Expressing Eimeria maxima Antigen Protects Chickens against Infection of the Two Parasites.
复制标题
表达最大艾美耳球虫抗原的尖复门艾美耳球虫的新型疫苗递送模型可保护鸡免受两种寄生虫的感染
DOI：
10.3389/fimmu.2017.01982
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发表时间：
2017
影响因子：
7.3
通讯作者：
Suo X
中科院分区：
文献类型：
--
作者：
Tang X;Liu X;Yin G;Suo J;Tao G;Zhang S;Suo X
疫苗递送对于抗原发现以及疫苗功效和安全性至关重要。人类和牲畜传染病的多样性需要开发不同的运载工具来针对不同的病原体。在家畜中，以前开发球虫病疫苗的策略遇到了一些障碍，限制了多种物种疫苗制剂的开发。在这里，我们描述了一种使用转基因艾美耳球虫表达最大艾美耳球虫免疫显性抗原的新型疫苗递送系统。在该递送系统中，巨型艾美球虫的免疫图谱蛋白 1 (EmIMP1) 在整个内源生命周期中由密切相关的柔嫩艾美球虫物种递送至宿主免疫系统。外源抗原的过表达不干扰转基因艾美球虫的繁殖和免疫原性。使用转基因寄生虫进行免疫后，我们检测了 EmIMP1 和巨型艾美球菌卵囊抗原的特异性体液和细胞免疫反应。特别是，我们观察到用转基因柔嫩艾美球虫免疫的鸡对随后的巨型艾美球虫感染有部分保护作用。我们的结果表明，转基因艾美耳球虫寄生虫是理想的球虫抗原递送载体，代表了一种新型球虫病疫苗。此外，该模型有可能用于开发疟疾活子孢子疫苗，其中不同菌株的抗原可以在疫苗菌株中表达。
Vaccine delivery is critical in antigen discovery and vaccine efficacy and safety. The diversity of infectious diseases in humans and livestock has required the development of varied delivery vehicles to target different pathogens. In livestock animals, previous strategies for the development of coccidiosis vaccines have encountered several hurdles, limiting the development of multiple species vaccine formulations. Here, we describe a novel vaccine delivery system using transgenic Eimeria tenella expressing immunodominant antigens of Eimeria maxima. In this delivery system, the immune mapped protein 1 of E. maxima (EmIMP1) was delivered by the closely related species of E. tenella to the host immune system during the whole endogenous life cycle. The overexpression of the exogenous antigen did not interfere with the reproduction and immunogenicity of transgenic Eimeria. After immunization with the transgenic parasite, we detected EmIMP1’s and E. maxima oocyst antigens’ specific humoral and cellular immune responses. In particular, we observed partial protection of chickens immunized with transgenic E. tenella against subsequent E. maxima infections. Our results demonstrate that the transgenic Eimeria parasite is an ideal coccidia antigen delivery vehicle and represents a new type of coccidiosis vaccines. In addition, this model could potentially be used in the development of malaria live sporozoite vaccines, in which antigens from different strains can be expressed in the vaccine strain.