Axolotl Metabolism: Measuring Metabolic Rate.
Axolotl Metabolism: Measuring Metabolic Rate.
复制标题
蝾螈代谢：测量代谢率。
DOI：
10.1007/978-1-0716-2659-7_26
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Khurgel,Moshe
中科院分区：
文献类型：
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作者：
Khurgel,Moshe
破译代谢过程如何有助于控制干细胞增殖和分化对于理解再生机制至关重要。然而，关于蝾螈的新陈代谢仍然有很多未知之处，在它们的生命周期或各种实验条件下都没有详细研究。我们总结了代谢和呼吸测量的理论基础，并描述了一个封闭的呼吸测量系统来研究蝾螈的代谢能量学作为代谢的一个具体方面。将吸收后相当不活跃的动物放置在多探头呼吸计中24-48小时，使我们能够测量呼吸气体浓度的变化：氧气（大气和溶解）和二氧化碳，同时监测室水的温度和盐度（电导率）。呼吸测定数据用于计算氧摄入量和二氧化碳排出量，以估计动物在观察期间的代谢能量动态。这种方法为研究蝾螈代谢率的潜在波动创造了机会，因为它涉及24小时昼夜节律周期期间的呼吸气体动力学，以及在衰老期间，在不同的环境温度下，在截肢后再生和许多其他情况下，代谢能量管理的变化。
Deciphering how metabolic processes contribute to control of stem cell proliferation and differentiation is essential for understanding the mechanisms of regeneration. However, much is still unknown about axolotls’ metabolism, which has not been studied in detail over their lifespan or under varied experimental conditions. We summarize the theoretical underpinnings of metabolism and respirometry, and describe a closed respirometry system to investigate metabolic energetics in axolotls as a specific aspect of metabolism. Placement of post-absorptive, fairly inactive animals in the multiple-probe respirometer for 24–48 h allows us to measure changes in concentrations of respiratory gases: oxygen (atmospheric and dissolved) and carbon dioxide, while monitoring the temperature and salinity (conductivity) of the chamber’s water. Respirometry data are used to calculate oxygen intake and carbon dioxide output to estimate animal’s metabolic energy dynamics during the observation periods. This method creates opportunities for study of potential fluctuations in axolotls’ metabolic rate as it pertains to respiratory gases’ dynamics during 24-h circadian cycle, as well as examination of changes in metabolic energy management during aging, under varied environmental temperatures, during post-amputation regeneration and many other circumstances.