MR tracking of transplanted glial cells using poly-L-lysine-CF3
MR tracking of transplanted glial cells using poly-L-lysine-CF3
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DOI：
10.1016/j.neures.2006.06.011
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发表时间：
2006-10-01
影响因子：
2.9
通讯作者：
Tooyama, Ikuo
中科院分区：
文献类型：
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作者：
Masuda, Chiaki;Maki, Zyunichi;Tooyama, Ikuo
使用超顺磁性氧化铁（SPIO）的磁共振（MR）成像是监测活体动物中移植细胞的有力工具。然而，由于SPIO是阴性造影剂，因此已经探索了阳性造影剂。在这项研究中，我们研究了FITC标记的聚-L-赖氨酸-CF 3（PLK-CF 3）使用胶质细胞的可行性。将FITC标记的PLK-CF 3加入培养液中，可被神经母细胞瘤细胞和神经胶质细胞内化。浓度低于80 μ g/ml时未观察到毒性。移植到活体小鼠脑内的标记细胞MR图像呈阳性。结果表明，FITC标记的PLK-CF 3是一种有用的MR跟踪阳性造影剂。(c)2006年Elsevier爱尔兰有限公司和日本神经科学学会。All rights reserved.
Magnetic resonance (MR) imaging using super-paramagnetic iron oxides (SPIOs) is a powerful tool to monitor transplanted cells in living animals. Since, however, SPIOs are negative contrast agents, positive agents have been explored. In this study, we examined the feasibility of FITC-labeled poly-L-lysine-CF3 (PLK-CF3) using glial cells. FITC-labeled PLK-CF3 was easily internalized by neuroblastoma cells and glia as adding it into culture medium. No toxicity was seen at the concentration of less than 80 mu g/ml. MR images positively detected labeled cells transplanted in the brain of living mouse. The results indicate that FITC-labeled PLK-CF3 is a useful positive contrast agent for MR tracking. (c) 2006 Elsevier Ireland Ltd and the Japan Neuroscience Society. All rights reserved.