Gene expression profiles in Ciona intestinalis stigmatal cells: Insight into formation of the ascidian branchial fissures
Gene expression profiles in Ciona intestinalis stigmatal cells: Insight into formation of the ascidian branchial fissures
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DOI：
10.1002/dvdy.20657
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发表时间：
2006-02
影响因子：
2.5
通讯作者：
Aki Shimazaki;A. Sakai;M. Ogasawara
中科院分区：
文献类型：
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作者：
Aki Shimazaki;A. Sakai;M. Ogasawara
鳃裂是咽上皮上的一系列开口，是半索动物和脊索动物身体结构的特征。在海鞘中，这些开口称为柱头，是在幼体发育过程中在鳃囊中形成的。基于海鞘幼鱼中表达的约 1,500 个基因的多重整体原位杂交分析，鉴定出主要在柱头细胞中表达的 28 个基因。这些柱头基因的表达模式分为四个不同的类别。基于这些发现，我们已经能够证明柱头的外围区域至少由三个不同的区域组成。在柱头穿孔和分裂过程中，在非纤毛细胞中检测到 Dlk1 样基因的表达，并在椭圆形柱头的基部区域保持不变。在纤毛柱头细胞分化过程中，meichroacidin、tektin A1 和 tektin B1 直向同源物的表达表明，涉及精子分化的一些分子机制可能被招募用于柱头发育，反之亦然。纤毛的成分如α-微管蛋白和根素也在柱头细胞中表达。这些基因可能有助于进一步分析鳃裂的进化和海鞘柱头的发育。发育动力学 235:562–569, 2006。© 2005 Wiley-Liss, Inc.
Gill slits, a series of openings in the pharyngeal epithelium, are characteristic features of the hemichordate and chordate body plans. In ascidians, these openings, called stigmata, are formed in the branchial sac during juvenile development. Multiple whole‐mount in situ hybridization analyses based on approximately 1,500 genes expressed in Ciona intestinalis juveniles, identified 28 genes expressed predominantly in the stigmatal cells. Expression patterns of these stigmatal genes were classified into four different categories. On the basis of these findings, we have been able to show that the peripheral region of a stigma consists of at least three different regions. The expression of a Dlk1‐like gene was detected in nonciliated cells during the stigma perforation and division and was maintained in the basal region of the elliptical stigma. Expression of meichroacidin, tektin A1, and tektin B1 orthologs during the differentiation of the ciliated stigmatal cells suggests that some of the molecular mechanisms involved in sperm differentiation might be recruited for the stigma development, or vice versa. Components of the cilia such as alpha‐tubulin and rootletin were also expressed in the stigmatal cells. These genes might facilitate further analyses regarding the evolution of the branchial fissures and the development of the ascidian stigmata. Developmental Dynamics 235:562–569, 2006. © 2005 Wiley‐Liss, Inc.