A preliminary proteomic survey of the in vitro excretory/secretory products of fourth-stage larval and adult Teladorsagia circumcincta
A preliminary proteomic survey of the in vitro excretory/secretory products of fourth-stage larval and adult Teladorsagia circumcincta
复制标题
DOI：
10.1017/s0031182005009510
复制
发表时间：
2006-04-01
期刊：
影响因子：
2.4
通讯作者：
Knox, DP
中科院分区：
文献类型：
--
作者：
Craig, H;Wastling, JM;Knox, DP
蛋白质的性质。包括第四阶段幼虫（L4）和成虫Teladorsagia circumcincta的体外排泄/分泌产物（ES），尽管这种线虫在感染的绵羊和山羊的皱胃表面的腺体的细胞结构中诱导了部分与寄生虫ES有关的深刻变化，但在很大程度上是不确定的。本研究采用双向电泳技术分离了L_4和成体ES细胞的蛋白质组分，并将考马斯亮蓝染色后的主要蛋白条带从凝胶上切下，进行胰蛋白酶消化和质谱分析。所得的肽质量指纹被用于鉴定15个L4和13个成人ES蛋白。两种胚胎干细胞中均含有珠蛋白和代谢酶等多种蛋白质。单独的L4 ES含有硫氧还蛋白过氧化物酶，所有酶都可以使由宿主对寄生虫的炎症反应产生的自由基解毒，半胱氨酸蛋白酶可能有助于胃粘膜的渗透，以及2种不同的半乳糖凝集素可能影响细胞分化和形态发生。成体ES含有核苷二磷酸激酶同源物，这种酶与细胞变化有关，可影响液体分泌和杯状细胞脱粒。
The nature of the proteins. which comprise the in vitro excretory/secretory products (ES) of the fourth-stage larva (L4) and adult Teladorsagia circumcincta are largely undefined, despite the fact that this nematode induces profound changes, in part related to parasite ES, in the cellular architecture of the glands fining the abomasal Surface of infected sheep and goats. In this study, the protein components of L4 and adult ES were fractionated using I D gel electrophoresis and the major protein bands, detected by Coomassie blue staining, excised from the gel and Subjected to tryptic digest and subsequent mass spectrometric analysis. The resultant peptide mass fingerprints were used to identify 15 L4 and 13 adult ES proteins. Several proteins, such as globin and some metabolic enzymes, were present in both ES. L4 ES alone contained thioredoxin peroxidase, all enzyme that can detoxify free radicals resulting from host inflammatory responses to the parasite, a cysteine proteinase which may aid penetration of the gastric mucosa and 2 different galectins which may influence cell differentiation and morphogenesis. Adult ES contained a nucleoside diphosphate kinase homologue, an enzyme which has been linked to cellular changes and can affect liquid secretion and goblet cell degranulation.