Biomarker function and nutritional stoichiometry of neutral lipid fatty acids and amino acids in oribatid mites
Biomarker function and nutritional stoichiometry of neutral lipid fatty acids and amino acids in oribatid mites
复制标题
DOI：
10.1016/j.soilbio.2017.07.020
复制
发表时间：
2017-12-01
影响因子：
9.7
通讯作者：
Heethofr, Michael
中科院分区：
文献类型：
--
作者：
Brueckner, Adrian;Hilpert, Andrea;Heethofr, Michael
生物标志物（例如脂肪酸、氨基酸、稳定同位素和分子条形码）对于研究土壤生态系统中的食物网结构和养分流动变得越来越重要。虽然脂肪酸的生物标志物功能已被调查的一些土壤动物类群（如跳虫和线虫），其作用在土壤中居住甲螨仍然未知。在这里，我们调查的生物标志物功能和营养化学计量的中性脂质脂肪酸（NLFA）和氨基酸甲螨。我们饲养的机会主义模型甲螨物种Archegozetes longisetosus的动物，细菌，真菌和草药来源的10个不同的资源。分别采用气相色谱/质谱联用（GC/MS）和离子交换色谱（IEC）分析了资源和消费者的中性脂质脂肪酸和氨基酸组成。我们发现饮食依赖的数量和组成的甲螨NLFA，但氨基酸是稳定的，独立的饮食。消费者NLFA组成可以用作使用数据挖掘方法的饮食的可靠预测器（即，随机森林），而氨基酸谱反映了饮食独立的内在生理特性，并证实了甲螨的稳态蛋白质化学计量假说。(C)2017爱思唯尔有限公司版权所有
Biomarkers (e.g. fatty acids, amino acids, stable isotopes, and molecular barcodes) have become increasingly important for investigating food web structure and nutrient flow in soil ecosystems. While the biomarker function of fatty acids has been investigated for some soil animal taxa (e.g. collembolans and nematodes), their role in soil-dwelling oribatid mites remained unknown. Here, we investigate the biomarker function and nutritional stoichiometry of neutral lipid fatty acids (NLFA) and amino acids in oribatid mites. We reared the opportunistic model oribatid mite species Archegozetes longisetosus on ten different resources of animal, bacterial, fungal and herbal origin. We analyzed the neutral lipid fatty acid and amino acid compositions of resources and consumers with gas chromatography/mass spectrometry (GC/MS) and ion-exchange chromatography (IEC), respectively. We found diet-dependent amounts and compositions of NLFA in the oribatid mites, but amino acids were stable and independent of diet. Consumer NLFA composition could be used as a reliable predictor of diet using data mining approaches (i.e., Random Forest), while amino acid profiles reflected diet-independent intrinsic physiological properties and confirm the homeostatic protein stoichiometry hypothesis for oribatid mites. (C) 2017 Elsevier Ltd. All rights reserved.