Transcription through the roadblocks:: the role of RNA polymerase cooperation
Transcription through the roadblocks:: the role of RNA polymerase cooperation
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DOI：
10.1093/emboj/cdg452
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发表时间：
2003-09-15
期刊：
影响因子：
11.4
通讯作者：
Nudler, E
中科院分区：
文献类型：
--
作者：
Epshtein, V;Toulmé, F;Nudler, E
在转录过程中，细胞RNA聚合酶（RNAP）必须处理由各种DNA结合蛋白施加的许多潜在障碍。许多这样的蛋白质在体外部分或完全中断RNA链延长的单轮。在这里，我们证明，大肠杆菌RNAP可以有效地阅读通过在体外和体内的位点特异性DNA结合蛋白，如果一个以上的RNAP分子被允许从相同的启动子启动。尾随RNAP的抗路障活性不需要转录物切割活性，而是依赖于路障复合物的正向易位。这些结果支持了一种合作的转录模型，即RNAP分子表现为“合作伙伴”，帮助彼此穿越内在和外在的障碍。
During transcription, cellular RNA polymerases (RNAP) have to deal with numerous potential roadblocks imposed by various DNA binding proteins. Many such proteins partially or completely interrupt a single round of RNA chain elongation in vitro. Here we demonstrate that Escherichia coli RNAP can effectively read through the site-specific DNA-binding proteins in vitro and in vivo if more than one RNAP molecule is allowed to initiate from the same promoter. The anti-roadblock activity of the trailing RNAP does not require transcript cleavage activity but relies on forward translocation of roadblocked complexes. These results support a cooperation model of transcription whereby RNAP molecules behave as 'partners' helping one another to traverse intrinsic and extrinsic obstacles.