Studies on the characteristics and mechanism of aerobic biodegradation of tetrabromobisphenol A by Irpex lacteus F17
Studies on the characteristics and mechanism of aerobic biodegradation of tetrabromobisphenol A by Irpex lacteus F17
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DOI：
10.1002/jobm.202000732
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发表时间：
2021-03
影响因子：
3.1
通讯作者：
Jie Chen;Juan Wu;Luosheng Fan;R. Jia
中科院分区：
文献类型：
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作者：
Jie Chen;Juan Wu;Luosheng Fan;R. Jia
研究了Irpex lacteus F17 （I. lacteus F17）在四种不同代谢底物（苯酚、葡萄糖、丙酮酸钠和柠檬酸钠）下对四溴双酚A （TBBPA）的好氧降解特性。乳酸菌F17对TBBPA的生物降解可通过共代谢增强，葡萄糖（8 g/L）为最佳碳源。结果表明，在初始溶液pH 3.0 ~ 8.0范围内，乳杆菌F17可在pH 4.0 ~ 8.0的较宽范围内降解TBBPA，降解率最高可达75.31%，在pH 5.0条件下，脱溴率最高可达12.40%。此外，还证实了Mn2+ (50 μmol/L)能促进锰过氧化物酶的分泌，提高TBBPA的生物降解效率。通过气相色谱-质谱分析鉴定了7个中间体，并提出了可能的降解途径，表明TBBPA的生物降解可能发生脱溴、β -裂解、羟基化、去质子化和氧化反应。
The study investigated the characteristics of aerobic degradation of tetrabromobisphenol A (TBBPA) by Irpex lacteus F17 (I. lacteus F17) under four different cometabolic substrates (phenol, glucose, sodium pyruvate, and sodium citrate). The biodegradation of TBBPA by I. lacteus F17 could be enhanced via cometabolism, and glucose (8 g/L) was confirmed to be the optimum carbon source. For different initial solution pH ranging from 3.0 to 8.0, the results showed that I. lacteus F17 could be applied to biodegrade TBBPA in a wide pH range of 4.0–8.0, and the degradation rate could reach the maximum 75.31%, while the debromination rate reached the maximum 12.40% under pH 5.0. In addition, it has been confirmed that Mn2+ (50 μmol/L) could promote the secretion of manganese peroxidase and TBBPA biodegradation efficiency. Seven intermediates were identified by gas chromatography–mass spectrometry analysis, and the possible degradation pathways were proposed, which indicated the biodegradation of TBBPA might be subjected to debromination, β‐scission, hydroxylation, deprotonation, and oxidation reactions.