M gene analysis of atypical strains of feline and canine coronavirus circulating in an Austrian animal shelter
M gene analysis of atypical strains of feline and canine coronavirus circulating in an Austrian animal shelter
复制标题
DOI：
10.1136/vr.159.6.170
复制
发表时间：
2006-08-05
影响因子：
2.2
通讯作者：
Moestl, K.
中科院分区：
文献类型：
--
作者：
Benetka, V.;Kolodziejek, J.;Moestl, K.
从奥地利动物收容所收集的12只猫和10只狗的粪便中收集的冠状病毒阳性样品通过逆转录酶PCR和序列分析进行了分析。此外，使用区分猫冠状病毒（Kov）I型和II型的引物对样品进行S基因分型。对M基因序列的系统发育分析表明，犬冠状病毒（CCoV）在犬中、典型的CCoV序列和最近描述的FCoV样CCoV中有明显的分离，而且猫中至少有两个FCoV的遗传簇，一个是种特异性的，另一个与FCoV样CCoV更密切相关。这些新的猫科病毒株的M基因序列与FCoV样CCoV 259/01株的同一性最高为88%，与GenBank中的任何FCoV序列的同一性最高仅为85%。在系统发育树中，它们位于猫和犬冠状病毒之间的中间位置。
Coronavirus-positive samples of faeces collected in an Austrian animal shelter from 12 cats and 10 dogs were analysed by reverse transcriptase-PCR With primers amplifying a segment of the M protein gene, and by sequence analysis. in addition, the samples were subjected to S gene typing, using primers that differentiated between feline coronavirus (Kov) types I and II. A phylogenetic analysis of the M gene sequences revealed not only clearly segregating canine coronavirus (CCoV) in the dogs, typical CCoV sequences and the recently described FCoV-Iike CCoV, but also at least two genetic clusters Of FCoV in the cats, one species-specific, the other more closely related to FCoV-Iike CCoV. The M gene sequences of these new feline strains had at most 88 per cent identity with the FCoV-like CCoV strain 259/01 and only up to 85 per cent with any FCoV sequence available in GenBank. In the phylogenetic tree they occupy an intermediate position between feline and canine coronaviruses.