GLUCAN-ENHANCED IMMUNOGENICITY OF KILLED ERYTHROCYTIC STAGES OF PLASMODIUM-BERGHEI
GLUCAN-ENHANCED IMMUNOGENICITY OF KILLED ERYTHROCYTIC STAGES OF PLASMODIUM-BERGHEI
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DOI：
10.1128/iai.32.2.542-546.1981
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发表时间：
1981-01-01
影响因子：
3.1
通讯作者：
PARKER, BW
中科院分区：
文献类型：
--
作者：
HOLBROOK, TW;COOK, JA;PARKER, BW
与单独注射灭活的红细胞期伯氏疟原虫相比，同时静脉注射葡聚糖和福尔马林灭活的红细胞期伯氏疟原虫在小鼠中引起了更大程度的抵抗随后感染活寄生虫的能力。在2项伯氏疟原虫NK 65菌株实验中，100%接种葡聚糖死亡寄生虫制剂的小鼠攻毒后存活;仅接种死亡寄生虫的小鼠存活率为28.6%。在第三个实验中，使用伯氏疟原虫菌株NYU-2，在用葡聚糖和死寄生虫免疫后存活的小鼠比例与单独用死寄生虫免疫后相同（即，每组11只中的10只），但是用葡聚糖-死寄生虫制剂免疫的小鼠比在感染前仅接受死寄生虫的小鼠经历显著更低强度和更短持续时间的制剂寄生虫血症。单独接种葡聚糖或与正常红细胞一起接种，对攻击没有保护作用。
I.v. injections of glucan simultaneously with Formalin-killed erythrocytic stages of P. berghei elicited a greater degree of resistance in mice against subsequent infection with viable parasites than injections of killed erythrocytic stages alone. In 2 experiments with P. berghei strain NK 65, 100% of mice immunized with the glucan-dead parasite preparation survived challenge; only 28.6% of mice receiving dead parasites alone survived. In the 3rd experiment, using P. berghei strain NYU-2, the same proportion of mice survived after immunization with glucan and dead parasites as with dead parasites alone (i.e., 10 of 11 in each group), but mice immunized with the glucan-dead parasite preparation experienced preparation parasitemias of significantly less intensity and shorter duration than mice which received only dead parasites before infection. Inoculation of glucan alone or with normal erythrocytes conferred no protection against challenge.