Interaction with free beta' subunit unmasks DNA-binding domain of RNA polymerase sigma subunit.
Interaction with free beta' subunit unmasks DNA-binding domain of RNA polymerase sigma subunit.
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与游离 β 亚基的相互作用揭示了 RNA 聚合酶 σ 亚基的 DNA 结合域。
DOI：
10.1016/s0014-5793(99)00778-4
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发表时间：
1999
期刊：
影响因子：
3.5
通讯作者：
Nikiforov,V
中科院分区：
文献类型：
--
作者：
Kulbachinskiy,A;Mustaev,A;Goldfarb,A;Nikiforov,V
除非σ 70与RNA聚合酶（RNAP）的核心成分结合，否则σ 70起始因子上的启动子识别位点被屏蔽而不与DNA相互作用。结果表明，σ 70与大肠杆菌RNAP β′亚基的相互作用足以使DNA结合位点的屏蔽解除。利用紫外线诱导的DNA-蛋白质交联，我们证明游离β′刺激σ 70多肽的区域2和含有TATAAT序列的非模板启动子链片段之间的特异性交联。因此，RNAP的σβ′亚组可以独立于RNAP核心的体积而呈现功能上胜任的构象。
The promoter recognition site on the σ70initiation factor is shielded from interaction with DNA unless σ70is bound to the core component of RNA polymerase (RNAP). It is shown that interaction of σ70with the isolated β′ subunit of Escherichia coli RNAP is sufficient to induce unshielding of the DNA binding site. Using UV-induced DNA-protein cross-linking we demonstrate that free β′ stimulates specific cross-links between region 2 of the σ70polypeptide and a fragment of the non-template promoter strand containing the TATAAT sequence. Thus the σβ′ subassembly of RNAP can assume a functionally competent conformation independently of the bulk of the RNAP core.