Untargeted metabolite profiling of liver in mice exposed to 2-methylfuran
Untargeted metabolite profiling of liver in mice exposed to 2-methylfuran
复制标题
暴露于 2-甲基呋喃的小鼠肝脏的非目标代谢物分析
DOI：
10.1111/1750-3841.15549
复制
发表时间：
2020-12-14
影响因子：
3.9
通讯作者：
Sun, Baoguo
中科院分区：
文献类型：
--
作者：
Hu, Chuanqin;Li, Ren;Sun, Baoguo
呋喃是一种重要的食物污染物，在许多熟食中发现。在大多数熟食中，呋喃被发现与一些烷基化衍生物如2-甲基呋喃共存。2-甲基呋喃被发现是有效的肝毒素。2-甲基呋喃的毒理学数据很少。本研究的目的是通过非靶向代谢组学方法研究2-甲基呋喃喂养小鼠肝脏样品中代谢物的变化。采用气相色谱-质谱联用（GC-MS）进行代谢组学分析。24种代谢产物被鉴定为差异代谢产物。重要的代谢途径是亚油酸代谢、甘氨酸、丝氨酸和苏氨酸代谢、甲烷代谢、抗坏血酸和乙醛酸代谢、缬氨酸、亮氨酸和异亮氨酸生物合成、花生四烯酸代谢、丙氨酸、天冬氨酸和谷氨酸代谢、氨酰-tRNA生物合成、半胱氨酸和蛋氨酸代谢、磷酸肌醇代谢和丙酮酸代谢。这些新发现的途径为研究2-甲基呋喃的毒性机制提供了依据。呋喃在食品中的实际应用已引起公众对啮齿类动物肝脏毒性和肝脏致癌性的关注。建立代谢组学方法，寻找更多的生物标志物来研究2-甲基呋喃的肝毒性机制。为制定食品中2-甲基呋喃的限量标准提供了重要信息。
Furan, a significant food contaminant, was found in many cooked foods. In most cooked foods, furan has been found to be coexisted with some alkylated derivatives such as 2-methylfuran. 2-methylfuran was found to be potent hepatotoxins. Little toxicological data is available for 2-methylfuran. The objective of this study was to investigate metabolite changes in the liver samples from mice fed with 2-methylfuran by untargeted metabolomic approach. Metabolomic analysis was conducted by using gas chromatography coupled with mass spectrometry (GC-MS). Twenty-four metabolites were identified as differential metabolites. The important metabolic pathway was linoleic acid metabolism, glycine, serine, and threonine metabolism, methane metabolism, ascorbate, and aldarate metabolism, valine, leucine, and isoleucine biosynthesis, arachidonic acid metabolism, alanine, aspartate, and glutamate metabolism, aminoacyl-tRNA biosynthesis, cysteine, and methionine metabolism, inositol phosphate metabolism, and pyruvate metabolism. These newly identified pathways provide evidence for investigating toxic mechanism of 2-methylfuran.Practical Application Furan in foods has caused public health concern for its hepatotoxicity and hepatic carcinogenicity in rodents. The metabolomics method was constructed to find more biomarkers to study underlying hepatotoxic mechanisms of 2-methylfuran. It will offer important information for official limits of 2-methylfuran in foods.