DRUG ASSAY USING ANTIBODY MIMICS MADE BY MOLECULAR IMPRINTING
DRUG ASSAY USING ANTIBODY MIMICS MADE BY MOLECULAR IMPRINTING
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DOI：
10.1038/361645a0
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发表时间：
1993-02-18
期刊：
影响因子：
64.8
通讯作者：
MOSBACH, K
中科院分区：
文献类型：
--
作者：
VLATAKIS, G;ANDERSSON, LI;MOSBACH, K
配体结合试验用于测定血流中微量物质。此类测定需要特异性结合感兴趣物质的受体。所使用的受体通常是抗体1 - 5，但抗体需要特殊处理和昂贵的生产程序5。我们已经使用分子印迹，一种在合成聚合物中产生选择性识别位点的方法6 - 8，来制备模拟抗体结合位点的聚合物。针对茶碱9和地西泮10的分子印迹显示出与抗体类似的强结合和交叉反应性。在这里，我们描述了一种新的放射性标记的配体结合测定，分子印迹吸附测定，它使用抗体模拟物。该测定法可准确测量人血清中的药物水平，结果与使用成熟的免疫测定技术获得的结果相当。抗体模拟物，其是稳定的，易于通过分子印迹制备，可以提供一个有用的一般替代抗体。
LIGAND-BINDING assays are used for determination of minute amounts of substances in the bloodstream. Such assays require a receptor that specifically binds the substance of interest. The receptor used is often an antibody1-5, but antibodies require special handling and a costly production procedure5. We have used molecular imprinting, a method for creating selective recognition sites in synthetic polymers6-8, to prepare polymers that mimic antibody combining sites. Molecular imprints made against theophylline9 and diazepam10 showed strong binding and cross-reactivity profiles similar to those of antibodies. Here we describe a new radiolabelled ligand-binding assay, the molecularly imprinted sorbent assay, which uses antibody mimics. This assay accurately measures drug levels in human serum, with results comparable to those obtained using a well established immunoassay technique. Antibody mimics, which are stable and readily prepared by molecular imprinting, may provide a useful general alternative to antibodies.