Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1
Role of plnB gene in the regulation of bacteriocin production in Lactobacillus paraplantarum L-XM1
复制标题
plnB基因在副植物乳杆菌L-XM1细菌素产生调控中的作用
DOI：
10.1016/j.micres.2012.11.008
复制
发表时间：
2013-01-01
影响因子：
6.7
通讯作者：
Li, Pinglan
中科院分区：
文献类型：
--
作者：
Zhang, Xiangmei;Shang, Nan;Li, Pinglan
Homologues of plnB gene have been shown to participate in regulation of bacteriocin production through quorum sensing system in other organisms, to investigate the possible role of plnB gene in Lactobacillus paraplantarum L-XM1, we cloned and insertionally inactivated the plnB gene. The plnB knockout mutant Delta plnB21 showed loss of bacteriocin production, its Bac(+) phenotype could not be restored even after the addition of PlnA. Furthermore, reverse transcription-PCR analysis from total RNA preparations showed that the bacteriocin structural genes of the plnEF and plnJK were not transcribed in the plnB knockout mutant compared with the wild-type strain. It was therefore concluded that plnB is invovled in a quorum sensing based bacteriocin production. This is the first demonstration of a role for plnB by gene knockout in L paraplantarum. (c) 2012 Elsevier GmbH. All rights reserved.
