Antioxidative activity of the hydrolytic enzyme treated Sorbus commixta Hedl. and its inhibitory effect on matrix metalloproteinase-1 in UV irradiated human dermal fibroblasts
Antioxidative activity of the hydrolytic enzyme treated Sorbus commixta Hedl. and its inhibitory effect on matrix metalloproteinase-1 in UV irradiated human dermal fibroblasts
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DOI：
10.1007/bf02980246
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发表时间：
2007-09-01
影响因子：
6.7
通讯作者：
Lee, Bum-Chun
中科院分区：
文献类型：
--
作者：
Bae, Jun-Tae;Sim, Gwan-Sub;Lee, Bum-Chun
超氧自由基。测定其清除DPPH自由基的活性和清除DPPH自由基的活性，以评价其抗氧化作用。提取(SCOE)。SCOE还用几种碳水解酶处理，显著增加了SCOE的总酚和类黄酮类成分。然后对酶处理后的SCOE进行抗氧化活性评估。SCOE经β-葡聚糖酶处理后，清除自由基活性最强。经β-葡聚糖酶处理的SCOE(β-GSCoE)具有清除自由基的活性，可增强紫外线照射下人真皮成纤维细胞(HDFS)的活力。用UVB(20mJ/cm(2))辐照的HDFS检测β-GSCoE对细胞内ROS的清除活性。用5-(6-)chloromethyl-2‘，7’dichlorodihydrofluorescein二乙酸酯荧光指示剂(CM-H(2)DCFDA)测定，紫外线照射可增加二氯荧光素的荧光强度。在含有β-GSCoE的培养液中，DCF荧光显著降低，表明β-GSCoE具有清除自由基的作用。通过检测UVA照射后HDFS中基质金属蛋白酶-1(MMP1)的表达，进一步验证了其保护作用。经UVA照射后，经β-GSCoE处理的HDFS中，基质金属蛋白酶-1蛋白和信使核糖核酸表达水平呈剂量依赖性下降。这些结果表明，β-GSCoE可能通过减少紫外线诱导的不良皮肤反应来减轻皮肤光老化的影响。
Superoxide radical. scavenging activity and DPPH radical scavenging activity were assessed in order to evaluate the antioxidant effect of the Sorbus commixta Hedl. extract (SCoE). SCoE was also treated with several carbohydrate-hydrolytic enzymes that significantly increased the total phenol and flavonoid composition of SCoE. The enzymatically treated SCoE was then assessed for antioxidative activity. The most efficient radical scavenging activity was observed when SCoE was treated with -glucanase. The radical scavenging activity of beta-glucanase-treated SCoE (beta-GSCoE) enhanced the viability of human dermal fibroblasts (HDFs) exposed to ultraviolet (UV) light. The intracellular reactive oxygen species (ROS) scavenging activity of beta-GSCoE was assessed using UVB (20 mJ/cm(2))-irradiated HDFs. UVB irradiation increased dichlorofluorescein (DCF) fluorescence, which was measured by a 5-(6-)chloromethyl-2',7'dichlorodihydrofluorescein diacetate (CM-H(2)DCFDA). DCF-fluorescence was significantly decreased in the beta-GSCoE-containing culture medium, suggesting that beta-GSCoE scavenges free radicals. The protective effect was further verified by assessing the expression of matrix meialloproteinase-1 (MMP-1) in UVA-irradiated HDFs. The treatment of UVA-irradiated HDFs with beta-GSCoE resulted in a dose-dependent decrease in the expression level of MMP-1 protein and mRNA. These results suggest that beta-GSCoE may mitigate the effects of photoaging in skin by reducing UV-induced adverse skin reactions.