Effect of D-Alanine in Teichoic Acid from the Streptococcus thermophilus Cell Wall on the Barrier-Protection of Intestinal Epithelial Cells
Effect of D-Alanine in Teichoic Acid from the Streptococcus thermophilus Cell Wall on the Barrier-Protection of Intestinal Epithelial Cells
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DOI：
10.1271/bbb.110646
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发表时间：
2012-02-01
影响因子：
1.6
通讯作者：
Tanabe, Soichi
中科院分区：
文献类型：
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作者：
Miyauchi, Eiji;Morita, Maho;Tanabe, Soichi
磷壁酸（TA）的D-丙氨酰化影响革兰氏阳性菌的各种功能，包括免疫调节作用。我们在这项研究中研究了来自嗜热链球菌ATCC 19258（T）的TA中的D-丙氨酸（D-Ala）对人肠Caco-2细胞屏障保护作用的影响。ATCC 19258（T）抑制肿瘤坏死因子-α诱导的跨上皮电阻（TER）（屏障功能的指标）降低。ATCC 19258（T）中TA的D-丙氨酰化具有生长期和培养温度依赖性。用Mg ~（2+）处理ATCC 19258（T）可降低TA中dlt mRNA的表达和DAla含量，并消除对TER降低的抑制作用。向培养液中补充L-丙氨酸（L-Ala）导致ATCC 19258（T）中L-Ala的增加和肠屏障保护作用的增加。因此，TA中的D-Ala在S.外源性L-Ala可增强这些有益作用。
D-Alanylation of teichoic acid (TA) affects various functions of Gram-positive bacteria, including immunomodulatory effects. We investigated in this study the impact of D-alanine (D-Ala) in TA from Streptococcus thermophilus ATCC 19258(T) on the barrier-protecting effect in human intestinal Caco-2 cells. ATCC 19258(T) suppressed the tumor necrosis factor-alpha-induced decrease in transepithelial electrical resistance (TER), an indicator of the barrier function. The D-alanylation of TA in ATCC 19258(T) was growth phase- and culture temperature-dependent. Treatment of ATCC 19258(T) with Mg2+ decreased the dlt mRNA expression and DAla content in TA and also abolished the suppressive effect on the TER decrease. Supplementation with L-alanine (L-Ala) to the broth led to an increase of L-Ala in ATCC 19258(T) and of the intestinal barrier-protecting effect. Taken together, D-Ala in TA played an important role in the barrier-protecting effect of S. thermophilus in the intestinal epithelium, and these beneficial effects could be enhanced by exogenous L-Ala.