Diagnostic and prognostic impact of six circulating microRNAs in acute coronary syndrome
Diagnostic and prognostic impact of six circulating microRNAs in acute coronary syndrome
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DOI：
10.1016/j.yjmcc.2011.07.011
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发表时间：
2011-11-01
影响因子：
5
通讯作者：
Thum, Thomas
中科院分区：
文献类型：
--
作者：
Widera, Christian;Gupta, Shashi K.;Thum, Thomas
循环microRNAs可能具有诊断急性冠脉综合征（ACS）的潜力。然而，以往的研究基于较少的患者数量，无法评估microrna与临床特征的关系及其潜在的预后价值。因此，我们在一个更大的ACS队列中评估了心肌细胞富集microrna在临床变量和敏感肌坏死生物标志物背景下的诊断和预后价值。采用定量反转录PCR方法检测444例ACS患者入院时血浆样本中MiR-1、miR-133a、miR-133b、miR-208a、miR-208b和miR-499的浓度。采用免疫分析法检测高灵敏度肌钙蛋白T （hsTnT）。随访6个月，观察全因死亡率。在包含临床变量和hsTnT的多元线性回归分析中，miR-1、miR-133a、miR-133b和miR-208b与hsTnT水平独立相关（P < 0.05）
Circulating microRNAs may have diagnostic potential in acute coronary syndrome (ACS). Previous studies, however, were based on low patient numbers and could not assess the relation of microRNAs to clinical characteristics and their potential prognostic value. We thus assessed the diagnostic and prognostic value of cardiomyocyte-enriched microRNAs in the context of clinical variables and a sensitive myonecrosis biomarker in a larger ACS cohort. MiR-1, miR-133a, miR-133b, miR-208a, miR-208b, and miR-499 concentrations were measured by quantitative reverse transcription PCR in plasma samples obtained on admission from 444 patients with ACS. High-sensitivity troponin T (hsTnT) was measured by immunoassay. Patients were followed for 6 months regarding all-cause mortality. In a multiple linear regression analysis that included clinical variables and hsTnT, miR-1, miR-133a, miR-133b, and miR-208b were independently associated with hsTnT levels (all P