Establishment and characterisation of new cell lines from human breast tumours initially established as tumour xenografts in NMRI nude mice
Establishment and characterisation of new cell lines from human breast tumours initially established as tumour xenografts in NMRI nude mice
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DOI：
10.1023/a:1005756632293
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发表时间：
1997-05-01
影响因子：
3.8
通讯作者：
Bibby, MC
中科院分区：
文献类型：
--
作者：
Hambly, RJ;Double, JA;Bibby, MC
需要人乳腺癌细胞系作为用于理解乳腺癌的模型，并用于提高细胞筛选检测适当的抗癌剂的能力。从在裸鼠中生长的人肿瘤异种移植物建立了四种人乳腺癌细胞系（MT-1、MaTu、MT-3和MC 4000）。所有的线被证明是人类起源的核型分析，上皮细胞的形态，光学和电子显微镜，细胞角蛋白18阳性，并从支原体，细菌，酵母菌和真菌污染。所有新细胞系均显示为ER和PgR阴性，而使用相同的程序（即放射性配体结合和免疫组织化学染色），阳性对照细胞系MCF-7显示为阳性。MaTu以前曾被报道为ER和PgR阳性的体内，它可能是，这一特点已经失去了由于在体外选择压力。所有新的乳腺癌细胞系的生长速率相似，并且在通过基于微量四唑的比色生长抑制测定法掺入用于筛选抗癌药物的组中所需的限度内。三个细胞系（MT-1、MaTu和MC 4000）也能够生长成肉眼可见的菌落，用于非琼脂克隆形成试验。此外，MT-1和MaTu在软琼脂培养基中均能形成球状体并具有克隆形成能力。新的细胞系对乳腺癌治疗中常用的抗癌药物表现出广泛的敏感性，并与相应的异种移植物一起为新化合物的评估提供了额外的系统。
Human breast cancer cell lines are required as models for use in the understanding of breast carcinoma, and for improving the ability of cell screens to detect appropriate anti-cancer agents. Four human breast cancer cell lines (MT-1, MaTu, MT-3 and MC4000) were established from human tumour xenografts grown in nude mice. All the lines were shown to be of human origin by karyotype analysis, were epithelial in morphology by both light and electron microscopy, were positive for cytokeratin 18, and were free from mycoplasma, bacterial, yeast and fungal contamination. All of the new lines were shown to be ER and PgR negative, while using the same procedures (i.e. radioligand binding and immunohistochemical staining) the positive control cell line MCF-7 was shown to be positive. MaTu had been previously reported as ER and PgR positive in vivo and it may be that this characteristic had been lost due to in vitro selection pressures. The growth rates of all the new breast cancer cell lines were similar and within the limits required for incorporation into a panel for screening anticancer drugs by a microtetrazolium based, colorimetric growth inhibition assay. Three of the lines (MT-1, MaTu and MC4000) were also able to grow into macroscopic colonies for use in a non-agar clonogenic assay. In addition, both MT-1 and MaTu formed spheroids and were clonogenic in soft-agar. The new lines demonstrated a wide range of sensitivities to anticancer agents commonly used in the treatment of breast cancer, and together with their corresponding xenografts are providing additional systems for the evaluation of new compounds.