Electrospraying: A Facile Technique for Synthesis of Chitosan-Based Micro/Nanospheres for Drug Delivery Applications
Electrospraying: A Facile Technique for Synthesis of Chitosan-Based Micro/Nanospheres for Drug Delivery Applications
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DOI：
10.1002/jbm.b.31085
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发表时间：
2009-01-01
影响因子：
3.4
通讯作者：
Katti, Dhirendra S.
中科院分区：
文献类型：
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作者：
Arya, Neha;Chakraborty, Syandan;Katti, Dhirendra S.
电喷雾是一种生成生物医学应用微/纳米球的新技术。除了是一种高产技术之外；电喷雾的另一个优点是不使用外部分散/乳液相，外部分散/乳液相通常涉及生物医学应用不需要的成分。在这项研究中，我们报告了使用电喷雾合成壳聚糖微/纳米球。重点是优化电喷雾的制造参数，以实现基于壳聚糖的微/纳米球的可重复合成，并研究它们作为生物活性剂递送载体的潜力。研究了以下因素的影响：(i)电喷雾电压、(ii)针距、(iii)壳聚糖溶液浓度、(iv)乙酸溶液浓度和(v)电喷雾距离。 SEM分析表明，当壳聚糖浓度为2%时，溶于90%乙酸中，可得到小于I pin的微球。产生良好结果的工作距离和针距分别为 7 厘米和 26 克。氨苄西林壳聚糖微纳米球的平均粒径为520μm，Zeta电位为+28.2mV，包封率为80.4%。对颗粒的药物释放动力学进行了表征，结果表明最初是爆发释放，随后在 120 小时内持续释放。此外，载药微/纳米球的抗菌活性表明，在电喷雾过程中暴露于高电压后，封装的药物仍处于其活性形式。这项研究表明，电喷雾是合成用于药物输送应用的壳聚糖微/纳米球的简便技术。 (c) 2008 年 Wiley periodicals, Inc. J Biomed Mater Res B 部分：Appl Biomater 8813：17-31，2009
Electrospraying is a novel technique for the generation of micro/nanospheres for biomedical applications. Apart from being a high yield technique; electrospraying has an added advantage of not making use of an external dispersion/emulsion phase which often involves ingredients that are undesirable for biomedical applications. In this study, we report the use of electrospraying for the synthesis of chitosan micro/nanospheres. The focus was to optimize the fabrication parameters involved in electrospraying for reproducible synthesis of chitosan based micro/nanospheres and to study their potential as delivery vehicles for bioactive agents. The influence of the following was studied (i) electrospraying voltage, (ii) needle gauge, (iii) concentration of chitosan solution, (iv) concentration of acetic acid solution, and (v) electrospraying distance. SEM analysis demonstrated that microspheres of less than I pin were obtained when chitosan concentration was 2% dissolved in 90% acetic acid. The working distance and needle gauge that yielded favorable results were 7 cm and 26 g, respectively. Average particle size of ampicillin loaded chitosan micro/nanospheres was 520 not with zeta potential of +28.2 mV and encapsulation efficiency of 80.4%. The particles were characterized for drug release kinetics and results demonstrated an initial burst release followed by a sustained release over a period of 120 h. Further, antibacterial activity of drug loaded micro/nanospheres demonstrated that the encapsulated drug was in its active form postexposure to high voltage during electrospraying. This study indicates that electrospraying is a facile technique for the synthesis of chitosan micro/nanospheres for drug delivery applications. (c) 2008 Wiley Periodicals, Inc. J Biomed Mater Res Part B: Appl Biomater 8813: 17-31, 2009