Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors
Non-enzymatic and highly sensitive lactose detection utilizing graphene field-effect transistors
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DOI：
10.1016/j.bios.2020.112419
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发表时间：
2020-10-01
影响因子：
12.6
通讯作者：
Sowwan, Mukhles
中科院分区：
文献类型：
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作者：
Danielson, Eric;Dindo, Mirco;Sowwan, Mukhles
基于低维材料的场效应晶体管(FET)生物传感器能够对各种分析物进行高灵敏度和特定的无标记检测。在这项工作中，我们制作了一种基于金纳米颗粒修饰的石墨烯的场效应管生物传感器，用于液门测量乳糖的检测。这种石墨烯装置带有人Galectin-3(hGal-3)蛋白的碳水化合物识别结构域(CRD)，可以通过凝集素-葡聚糖亲和结合在石墨烯上的供体效应来检测乳糖的存在。虽然乳糖的检测很重要，因为它普遍存在于食品中和疾病相关的应用(乳糖不耐受情况)，在这项工作中，我们利用凝集素/碳水化合物的相互作用来开发一种设备，原则上可以专门检测任何碳水化合物的极低浓度。该传感器对乳糖浓度的动态响应范围为1 FM~1 PM(10(-15)~10(-12)摩尔L-1)，检测下限为200 am，比以往的石墨烯电化学器件有了显著的提高。FET传感器对AM浓度的乳糖的响应也是特异的，这表明凝集素和石墨烯FET(G-FET)传感器在检测碳水化合物方面具有高灵敏度和高特异度的潜力，用于疾病诊断。
Field-effect transistor (FET) biosensors based on low-dimensional materials are capable of highly sensitive and specific label-free detection of various analytes. In this work, a FET biosensor based on graphene decorated with gold nanoparticles (Au NPs) was fabricated for lactose detection in a liquid-gate measurement configuration. This graphene device is functionalized with a carbohydrate recognition domain (CRD) of the human galectin-3 (hGal-3) protein to detect the presence of lactose from the donor effect of lectin - glycan affinity binding on the graphene. Although the detection of lactose is important because of its ubiquitous presence in food and for disease related applications (lactose intolerance condition), in this work we exploit the lectin/carbohydrate interaction to develop a device that in principle could specifically detect very low concentrations of any carbohydrate. The biosensor achieved an effective response to lactose concentrations over a dynamic range from 1 fM to 1 pM (10(-15) to 10(-12) mol L-1) with a detection limit of 200 aM, a significant enhancement over previous electrochemical graphene devices. The FET sensor response is also specific to lactose at aM concentrations, indicating the potential of a combined lectin and graphene FET (G-FET) sensor to detect carbohydrates at high sensitivity and specificity for disease diagnosis.