Probiotics Reduce the Inflammatory Response Induced by a High-Fat Diet in the Liver of Young Rats
Probiotics Reduce the Inflammatory Response Induced by a High-Fat Diet in the Liver of Young Rats
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DOI：
10.3945/jn.108.101808
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发表时间：
2009-05-01
影响因子：
4.2
通讯作者：
Meli, Rosaria
中科院分区：
文献类型：
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作者：
Esposito, Emanuela;Iacono, Anna;Meli, Rosaria
非酒精性脂肪肝（NAFLD）是儿科人群中最常见的慢性肝病形式。初步证据表明，益生菌对这种情况有潜在的治疗作用。在此，我们检测了益生菌VSL#3（一种由嗜热链球菌和几种乳杆菌和双歧杆菌组成的多菌株制剂）对幼龄大鼠肝脏中高脂饮食诱导的氧化和炎症损伤的潜在影响。在断奶时，将年轻的雄性Sprague-Dawley大鼠随机分为3组（n = 6），分别饲喂标准的非纯化饮食（Std; 5.5%的能量来自脂肪）或高脂流质饮食（HFD; 71%的能量来自脂肪）。其中一个HFD组通过灌胃给予VSLI #3（13 X 10（9）bacteria.kg（-1）.d（-1））。4周后，HFD大鼠的体重增加、脂肪量、血清转氨酶和肝脏重量均高于饲喂标准饲料的大鼠。HFD诱导肝脏脂质过氧化、肿瘤坏死因子（TNF α）产生、蛋白S-亚硝基化、诱导型一氧化氮合酶（NOS）、环氧合酶（考克斯）-2表达和金属蛋白酶（MMP）活性。此外，在HFD组中，过氧化物酶体增殖物激活受体α表达低于饲喂标准饲料的大鼠。在喂食HFD饲料并接受VSLI #3给药的大鼠中，肝脏TNF α水平、MMP-2和MMP-9活性以及iNOS和考克斯-2表达均显著低于HFD组。在VSLI #3给药大鼠中，PPAR α表达高于HFD组。还证明了VSLI #3对核因子-κ B通路的调节。我们的数据表明，VSLI #3给药可限制NAFLD患者的氧化性和炎症性肝损伤。J. Nutr. 139：905-911。2009.
Nonalcoholic fatty liver disease (NAFLD) is the most common form of chronic liver disease in the pediatric population. Preliminary evidence suggests a potential therapeutic utility of probiotics for this condition. Here, we tested the potential effect of the probiotic VSL#3 (a multistrain preparation composed of Streptococcus thermophilus and several species of Lactobacillus and Bifidobacteria) on oxidative and inflammatory damage induced by a high-fat diet in the liver of young rats. At weaning, young male Sprague-Dawley rats were randomly divided into 3 groups (n = 6) fed a standard, nonpurified diet (Std; 5.5% of energy from fat) or a high-fat liquid diet (HFD; 71% of energy from fat). One of the HFD groups received by gavage VSL#3 (13 X 10(9) bacteria.kg(-1).d(-1)). After 4 wk, the HFD rats had greater body weight gain, fat mass, serum aminotransferase, and liver weight than rats fed the Std diet. The HFD induced liver lipid peroxidation, tumor necrosis factor (TNF alpha) production, protein S-nitrosylation, inducible nitric oxide synthase (NOS), cyclooxygenase (COX)-2 expression, and metalloproteinase (MMP) activity. Moreover, in the HFD group, PPAR alpha expression was less than in rats fed the Std diet. In rats fed the HFD diet and treated with VSL#3, liver TNF alpha levels, MMP-2 and MMP-9 activities, and expression of iNOS and COX-2 were significantly lower than in the HFD group. In VSL#3-treated rats, PPAR alpha expression was greater than in the HFD group. A modulation of the nuclear factor-kappa B pathway by VSL#3 was also demonstrated. Our data suggest that VSL#3 administration could limit oxidative and inflammatory liver damage in patients with NAFLD. J. Nutr. 139: 905-911. 2009.