Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species
Molecular phylogenetic analysis of Cyclospora, the human intestinal pathogen, suggests that it is closely related to Eimeria species
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DOI：
10.1093/infdis/173.2.440
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发表时间：
1996-02-01
影响因子：
6.4
通讯作者：
Echeverria, P
中科院分区：
文献类型：
--
作者：
Relman, DA;Schmidt, TM;Echeverria, P
一种归属于环孢子虫属的球虫生物体已被越来越多地认识到与全世界人类的长期腹泻有关。尽管形态学和孢子形成特征可用，但围绕这种挑剔生物体的分类仍存在混乱。从人粪便标本中纯化的环孢子虫卵囊的小亚基rRNA编码区扩增并测序，相同的序列存在于来自其他8名患有环孢子虫卵囊的患者的标本中，但在来自无症状受试者和来自隐孢子虫病患者的标本中不存在。rDNA序列的系统发育分析揭示，人类相关的环孢子虫与艾美耳球虫属的成员密切相关，这些结果可以预测环孢子虫宿主特异性、生命周期和流行病学，以及开发基于特定聚合酶链反应的诊断检测方法。
A coccidian organism assigned to the genus Cyclospora has been increasingly recognized in association with prolonged diarrhea in humans throughout the world, Confusion surrounds the taxonomy of this fastidious organism, despite the availability of morphology and sporulation characteristics, The small subunit rRNA coding region from cyclosporan oocysts purified from a human fecal specimen was amplified and sequenced, The same sequence was present in specimens from 8 other patients with cyclosporan oocysts but absent in specimens from asymptomatic subjects and from cryptosporidiosis patients, Phylogenetic analysis of rDNA sequences reveals that the human-associated Cyclospora is closely related to members of the Eimeria genus, These results allow predictions concerning Cyclospora host specificity, life cycle, and epidemiology as well as the development of a specific polymerase chain reaction-based diagnostic assay.