Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells
Overexpression of human catalase inhibits proliferation and promotes apoptosis in vascular smooth muscle cells
复制标题
DOI：
10.1161/01.res.85.6.524
复制
发表时间：
1999-09-17
影响因子：
20.1
通讯作者：
Weintraub, NL
中科院分区：
文献类型：
--
作者：
Brown, MR;Miller, FJ;Weintraub, NL
活性氧的作用，如超氧阴离子（O-2（.-））为了研究内源性过氧化氢（H2 O2）的作用，用含有人过氧化氢酶（AdCat）cDNA或对照基因β-半乳糖苷酶（AdLacZ）的腺病毒载体感染大鼠主动脉平滑肌细胞。感染AdCat后，细胞内过氧化氢酶活性增加50 ~ 100倍，细胞内H2 O2浓度降低。在含有2%血清的培养基中，AdCat感染降低了细胞对[H-3]胸苷的摄取，这是DNA合成的一个指标（0.37+/-0.09解体/分钟/细胞[AdLacZ]对0.22+/-0.08解体/分钟/细胞[AdCat]，P
The role of reactive oxygen species, such as superoxide anions (O-2(.-)) nd hydrogen peroxide (H2O2), in modulating vascular smooth muscle cell proliferation and viability is controversial, To investigate the role of endogenously produced H2O2, rat aortic smooth muscle cells were infected with adenoviral vectors containing cDNA for human catalase (AdCat) or a control gene, beta-galactosidase (AdLacZ). Infection with AdCat resulted in dose-dependent increases in intracellular catalase protein, which was predominantly localized to peroxisomes, After infection with 100 multiplicity of infection (MOI) of AdCat, cellular catalase activity was increased by 50- to 100-fold, and intracellular H2O2 concentration was reduced, as compared with control. Infection with AdCat reduced [H-3]thymidine uptake, an index of DNA synthesis, in cells maintained in medium supplemented with 2% serum (0.37+/-0.09 disintegrations per minute per cell [AdLacZ] versus 0.22+/-0.08 disintegrations per minute per cell [AdCat], P