Determination of apparent amylose content, pasting properties and gel texture of rice starch by near-infrared spectroscopy
Determination of apparent amylose content, pasting properties and gel texture of rice starch by near-infrared spectroscopy
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DOI：
10.1002/jsfa.2960
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发表时间：
2007-08-30
影响因子：
4.1
通讯作者：
Shen, Yun
中科院分区：
文献类型：
--
作者：
Bao, Jinsong;Wang, Yuefei;Shen, Yun
为了选育优质稻米，可常规测定稻米的表观直链淀粉含量（AAC）、快速粘度分析仪（RVA）的糊化粘度和凝胶质构特性。由于直接测量既耗时又昂贵，因此基于近红外光谱（LAIRS）的快速预测方法对于测量这些质量参数非常有用。在这项研究中，校准模型的开发使用修改后的偏最小二乘回归与不同的数学处理的基础上的粮食和面粉光谱的非糯性大米单独或与糯性大米组合。结果表明，用面粉光谱建立的校正模型比用谷物光谱建立的校正模型更稳健，用含糯大米的全米建立的校正模型比用非糯大米建立的校正模型更具有上级优势。LAIRS基于面粉光谱可以较准确地预测AAC、消减粘度（SB）、糊化温度（PT）、硬度（HD）和粘聚性（COH）等淀粉品质参数，而基于籽粒光谱只能较准确地预测AAC和PT。本文报道的所有模型都可用于粗样品筛选（冷糊粘度和崩解粘度）、样品筛选（SB、PT和COH）以及大多数应用（AAC和HD），用于育种计划中早期世代选择中大量样品的常规筛选。然而，为了准确测定糊化粘度和凝胶质构参数，后代应采用直接仪器测量。(C)2007年化学工业协会
For breeding rice with improved quality, apparent amylose content (AAC), rapid visco analyser (RVA) pasting viscosities and gel texture properties may be routinely measured. As a direct measurement is time-consuming and expensive, rapid predictive method based on near-infrared spectroscopy (LAIRS) is useful for measurement of these quality parameters. In this study, calibration models were developed using modified partial least-squares regression with different mathematical treatments based on the grain and flour spectra of non-waxy rice alone or in combination with waxy rice. The results showed that calibration models built with flour spectra are more robust than those with grain spectra, and with total rice including waxy rice are superior to those with only non-waxy rice. Some starch quality parameters, such as AAC, setback viscosity (SB), pasting temperature (PT), hardness (HD) and cohesiveness (COH) could be predicted with sufficient accuracy by LAIRS based on flour spectra, whereas only AAC and PT could be predicted with sufficient accuracy based on grain spectra. All the models reported here are usable for rough sample screening (cold paste viscosity and breakdown viscosity), sample screening (SB, PT and COH) and for most applications (AAC and HD) for routine screening of a large number of samples in the early generation selection in breeding programs. However, for accurate assay of the pasting viscosity and gel textural parameters, direct instrumental measurement should be employed in later generations. (C) 2007 Society of Chemical Industry