Bacterial ecosystem functioning in organic matter biodegradation of different composting at the thermophilic phase

Bacterial ecosystem functioning in organic matter biodegradation of different composting at the thermophilic phase
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DOI:
10.1016/j.biortech.2020.123990
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发表时间:
2020-12-01
影响因子:
11.4
通讯作者:
Shen, Qirong
Shen, Qirong
中科院分区:
工程技术1区
文献类型:
--
作者:
Kong, Zhijian;Wang, Xuanqing;Shen, Qirong

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本研究旨在通过分析关键细菌种群和功能,为堆肥生态功能的预测提供参考。以牛粪、鸡粪、菇渣和中药渣为原料,通过合成加标标准品结合Illumina测序和PICRUSt,对细菌群落功能进行定量和预测。麦秆鸡粪堆肥(SCM)和菇渣鸡粪堆肥(MCM)的细菌群落结构相似,均以Sinibacillus为主,丰度分别为20.73%和41.36%。细菌群落与荧光EEM区域积分参数的相关性分析表明,乳酸杆菌(0.889)、肠球菌(0.888)和丹毒杆菌(0.903)与P-v,n / P-III,n.本体分析结果表明,代谢、遗传信息处理、环境信息处理和细胞过程是各处理下细菌群落的主要功能。
This study aimed to provide insights into prediction of composting ecological functioning through analyzing the critical bacterial populations and functions. The bacterial ecosystem functioning was essential, and cow dung, chicken manure, mushroom dreg and Chinese medicine residues were used as raw materials to quantify and predict the functioning of bacterial communities through synthetic spike-in standards accompanied Illumina sequencing and PICRUSt. Bacterial community of wheat straw and chicken manure compost (SCM) was similar to mushroom dreg and chicken manure compost (MCM), and Sinibacillus dominated in both treatments with the abundance of 20.73% and 41.36%, respectively. The correlation analysis between bacterial community and fluorescence EEM regional integration parameters showed that Lactobacillus (0.889), Enterococcus (0.888) and Erysipelothri (0.903) were positively correlated with P-v, n / P-III, n. The ontology analysis results showed that metabolism, genetic information processing, environmental information processing and cellular processes were the primary functions for bacterial community in all treatments.