Associations of gut-flora-dependent metabolite trimethylamine-N-oxide, betaine and choline with non-alcoholic fatty liver disease in adults.
Associations of gut-flora-dependent metabolite trimethylamine-N-oxide, betaine and choline with non-alcoholic fatty liver disease in adults.
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肠道依赖性代谢物三甲胺-N-氧化物、甜菜碱和胆碱与成人非酒精性脂肪肝的关系
DOI：
10.1038/srep19076
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发表时间：
2016-01-08
影响因子：
4.6
通讯作者：
Zhu HL
中科院分区：
文献类型：
--
作者：
Chen YM;Liu Y;Zhou RF;Chen XL;Wang C;Tan XY;Wang LJ;Zheng RD;Zhang HW;Ling WH;Zhu HL
许多研究表明，三甲胺- n-氧化物（TMAO），胆碱的肠道菌群依赖性代谢物，有助于心血管疾病的风险，但对非酒精性脂肪性肝病（NAFLD）知之甚少。我们研究了循环TMAO、胆碱和甜菜碱与中国成人NAFLD的存在和严重程度的关系。我们进行了一项基于医院的病例对照研究（CCS）和一项横断面研究（CSS）。在CCS中，我们招募了60例活检证实的NAFLD病例和35例对照（18-60岁），并通过HPLC-MS/MS测定了血清中TMAO、胆碱和甜菜碱的浓度。在CSS中，1,628名社区成人（40-75岁）完成了血液检查和超声检查NAFLD评估。在CCS中，协方差分析显示，血清TMAO、胆碱和甜菜碱/胆碱比值与脂肪变性评分和NAFLD总活性（NAS）呈负相关（p趋势均<0.05）。CSS显示NAFLD的严重程度与TMAO升高、甜菜碱和甜菜碱/胆碱比值降低独立相关（p趋势均<0.05）。未观察到胆碱与nafld的显著关联。我们的研究结果显示，在医院和社区为基础的中国成年人中，循环TMAO水平与NAFLD的存在和严重程度之间存在不良关联，而在社区为基础的参与者中，甜菜碱与NAFLD之间存在良好的关系。
Many studies suggest that trimethylamine-N-oxide (TMAO), a gut-flora-dependent metabolite of choline, contributes to the risk of cardiovascular diseases, but little is known for non-alcoholic fatty liver disease (NAFLD). We examined the association of circulating TMAO, choline and betaine with the presence and severity of NAFLD in Chinese adults. We performed a hospital-based case-control study (CCS) and a cross-sectional study (CSS). In the CCS, we recruited 60 biopsy-proven NAFLD cases and 35 controls (18–60 years) and determined serum concentrations of TMAO, choline and betaine by HPLC-MS/MS. For the CSS, 1,628 community-based adults (40-75 years) completed the blood tests and ultrasonographic NAFLD evaluation. In the CCS, analyses of covariance showed adverse associations of ln-transformed serum levels of TMAO, choline and betaine/choline ratio with the scores of steatosis and total NAFLD activity (NAS) (all P-trend <0.05). The CSS revealed that a greater severity of NAFLD was independently correlated with higher TMAO but lower betaine and betaine/choline ratio (all P-trend <0.05). No significant choline-NAFLD association was observed. Our findings showed adverse associations between the circulating TMAO level and the presence and severity of NAFLD in hospital- and community-based Chinese adults, and a favorable betaine-NAFLD relationship in the community-based participants.