ERYTHROCYTE ANKYRIN - IMMUNOREACTIVE ANALOGS ARE ASSOCIATED WITH MITOTIC STRUCTURES IN CULTURED-CELLS AND WITH MICROTUBULES IN BRAIN
ERYTHROCYTE ANKYRIN - IMMUNOREACTIVE ANALOGS ARE ASSOCIATED WITH MITOTIC STRUCTURES IN CULTURED-CELLS AND WITH MICROTUBULES IN BRAIN
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DOI：
10.1073/pnas.78.12.7550
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发表时间：
1981-01-01
期刊：
影响因子：
--
通讯作者：
DAVIS, J
中科院分区：
文献类型：
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作者：
BENNETT, V;DAVIS, J
人红细胞锚蛋白是血影蛋白的膜附着蛋白，通过放射免疫测定法在多种细胞和组织中检测到。在脑和[人宫颈癌] HeLa细胞中与锚蛋白交叉反应的多肽，并证明这些锚蛋白类似物的功能涉及与微管的关联。锚蛋白的免疫反应性被定位在一个秋水仙碱和洗涤剂敏感的细胞质网间期细胞的间接免疫荧光。也有特定的核染色，定位在亮点，这是完全由锚蛋白或高分子量微管相关蛋白（MAP）从大脑中取代。分裂期细胞核点状染色消失，网状结构消失。荧光定位在纺锤体极中期，并重新分配到分裂沟后期阶段的有丝分裂。在脑提取物中鉴定了与MAP 1共迁移的免疫反应性MW 370，000多肽，并通过重复的聚合和解聚循环与微管共聚。红细胞锚蛋白与从纯微管蛋白制备的微管相关;这种结合被脑MAPs取代。
Human erythrocyte ankyrin, the membrane attachment protein for spectrin, was detected by radioimmunoassay in a variety of cells and tissues. Polypeptides crossreacting with ankyrin in brain and [human cervical carcinoma] HeLa cells and demonstrates that 1 function of these ankyrin analogs involves association with microtubules. Ankyrin immunoreactivity was localized by indirect immunofluorescence in a colchicine- and detergent-sensitive cytoplasmic meshwork in interphase cells. There also was specific nuclear staining, localized in bright spots, which was displaced entirely by ankyrin or by high MW microtubule-associated-proteins (MAPs) from brain. In dividing cells, the punctate nuclear staining and the meshwork disappeared. Fluorescence was localized at the spindle pole during metaphase and was redistributed to the cleavage furrow in later stages of mitosis. An immunoreactive MW 370,000 polypeptide comigrating with MAP1 was identified in brain extracts and copolymerized with microtubules through repeated cycles of polymerization and depolymerization. Erythrocyte ankyrin associated with microtubules prepared from pure tubulin; this binding was displaced by brain MAPs.