Age-related changes in the glycation of human aortic elastin
Age-related changes in the glycation of human aortic elastin
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DOI：
10.1016/j.exger.2003.10.003
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发表时间：
2004-02-01
影响因子：
3.9
通讯作者：
Velkova, A
中科院分区：
文献类型：
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作者：
Konova, E;Baydanoff, S;Velkova, A
蛋白质的非酶糖化是糖尿病高血糖症的结果，与衰老相关。本研究的目的是通过两种方法研究健康人主动脉弹性蛋白糖基化的增龄性变化：（1）荧光法测定不同年龄组人主动脉弹性蛋白糖基化终末产物（AGEs）;（2）不同年龄组弹性蛋白的体外糖基化及其早期形成能力（氮蓝四唑比色法）和AGEs（荧光法）的研究。人不溶性弹性蛋白的制备从宏观和微观不变的地区，从68个事故受害者，分布在15个年龄组，使用Starcher和Galione的方法获得的胸腰椎。可溶性α-弹性蛋白是通过Parlard等人的方法获得的。年龄组中美拉德反应相关荧光的直接评估显示荧光随年龄增加。年轻的弹性蛋白具有最高的能力，形成果糖胺和糖基化的老年弹性蛋白的糖基化下没有显着增加在孵育过程中。这些结果与体外糖基化一致。由于其生物半衰期长，弹性蛋白易受缓慢糖化过程的影响，以下修饰将导致结缔组织的年龄相关变化。(C)2003年爱思唯尔公司All rights reserved.
Non-enzymatic glycation of proteins is a consequence of hyperglycemia in diabetes and correlates with aging. The aim of the study was to investigate age-related changes in the glycation of human aortic elastin in healthy subjects by two approaches: (1) assessment by fluorescence method of formed in vivo advanced glycation end products (AGEs) of elastins, purified from human aortas, obtained from different age groups;, (2) in vitro glycation of elastins from different age groups and investigation of their capacity to form early (by colorimetric nitroblue tetrazolium method) and AGEs (fluorescence method). Human insoluble elastins were prepared from macro- and microscopic unaltered regions of thoracic aortas, obtained from 68 accident victims, distributed in 15 age-groups, using the method of Starcher and Galione. Soluble alpha-elastins were obtained by the method of Partridge et al. The direct assessment of Maillard reaction related fluorescence in the age groups showed increase of the fluorescence with age. The 'young' elastin had the highest capacity to form both fructosamine and AGEs under glycation of 'old' elastin did not increase markedly during the incubation. These results are consistent with the glycation in vitro. The interpretation that because of its long biological half-life, elastin is susceptible to the slow process of glycation and the following modifications would contribute to the age-related changes of connective tissue. (C) 2003 Elsevier Inc. All rights reserved.