Characterization of OsPIP2;7, a Water Channel Protein in Rice
Characterization of OsPIP2;7, a Water Channel Protein in Rice
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水稻水通道蛋白 OsPIP2;7 的表征
DOI：
10.1093/pcp/pcn166
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发表时间：
2008-12-01
影响因子：
4.9
通讯作者：
Su, Wei-Ai
中科院分区：
文献类型：
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作者：
Li, Guo-Wei;Zhang, Min-Hua;Su, Wei-Ai
水通道蛋白是水通道蛋白，促进水和其他小的中性分子通过生物膜。高等植物中通常有大量的这一科成员，它们表现出各种生理功能，并在特定的时间和特定模式下受到调节。我们之前已经证明，水稻基因OsPIP2；7、在低温胁迫早期，在根中普遍表达上调，而在茎中表达下调。在这里,OsPIP2;经克隆证实，该蛋白是一种在爪蟾卵母细胞中具有较高活性的水通道蛋白。OsPIP2;7主要定位于叶片叶肉细胞。在根中，在伸长区维管组织、表皮细胞和外表皮细胞中，在成熟区表皮细胞、外表皮细胞和根毛中均检测到。酵母细胞过表达OsPIP2；7株冻融胁迫后存活率较高。此外,OsPIP2;7在水稻中过量表达可提高蒸腾速率和耐低温能力。结果表明，OsPIP2；7参与快速水分运输和维持细胞内水分平衡，最终提高酵母和水稻对低温胁迫的耐受性。
Aquaporins are water channel proteins that facilitate passage of water and other small neutral molecules across biological membranes. There are usually a large number of members of this family in higher plants, which exhibit various physiological functions and are regulated in a time-specific and particular mode. We have previously shown that a rice gene, OsPIP2;7, was generally up-regulated in roots but down-regulated in shoots at the early stage of chilling stress. Here, OsPIP2;7 was cloned and proved to be an aquaporin with high activity in Xenopus oocytes. OsPIP2;7 was localized mainly in mesophyll cells of leaves. In roots it was detected in the vascular tissues, epidermis cells and exodermis cells at the elongation zone, as well as in the epidermis cells, exodermis cells and root hair at the maturation zone. Yeast cells overexpressing OsPIP2;7 showed a higher survival rate after freezethaw stress. Furthermore, OsPIP2;7 enhanced the transpiration rate and tolerance to low temperature when overexpressed in rice. These results indicated that OsPIP2;7 was involved in rapid water transport and maintenance of the water balance in cells, and ultimately improves the tolerance of yeast and rice to low temperature stress.