Selenium incorporation using recombinant techniques.
Selenium incorporation using recombinant techniques.
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DOI：
10.1107/s0907444909038207
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发表时间：
2010-04
期刊：
影响因子：
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通讯作者：
Walden H
中科院分区：
文献类型：
--
作者：
Walden H
综述了硒的重组掺入以及随后的纯化和所得标记蛋白的结晶技术。沿着使用硒代半胱氨酸，使用硒代甲硫氨酸使大分子结构定相是结构测定中的常见实践。因此，硒是MAD最常用的重原子。除了在原核表达系统中引入硒的成熟重组技术外，在真核表达中硒标记的最新进展，将进行讨论。提示和事情考虑硒标记的蛋白质的纯化和结晶也包括在内。
An overview of techniques for recombinant incorporation of selenium and subsequent purification and crystallization of the resulting labelled protein. Using selenomethionine to phase macromolecular structures is common practice in structure determination, along with the use of selenocysteine. Selenium is consequently the most commonly used heavy atom for MAD. In addition to the well established recombinant techniques for the incorporation of selenium in prokaryal expression systems, there have been recent advances in selenium labelling in eukaryal expression, which will be discussed. Tips and things to consider for the purification and crystallization of seleno-labelled proteins are also included.