Antioxidant profiling of vine tea (Ampelopsis grossedentata): Off-line coupling heart-cutting HSCCC with HPLC-DAD-QTOF-MS/MS
Antioxidant profiling of vine tea (Ampelopsis grossedentata): Off-line coupling heart-cutting HSCCC with HPLC-DAD-QTOF-MS/MS
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藤茶（Ampelopsisgrosedentata）的抗氧化分析：使用 HPLC-DAD-QTOF-MS/MS 离线耦合中心切割 HSCCC
DOI：
10.1016/j.foodchem.2016.11.122
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发表时间：
2017-06-15
期刊：
影响因子：
8.8
通讯作者：
Xiang, Haiyan
中科院分区：
文献类型：
--
作者：
Gao, Qingping;Ma, Ruyi;Xiang, Haiyan
藤茶具有很强的抗氧化活性，通常作为健康茶/饮料消费。然而，关于其抗氧化剂的详细信息并不完整。采用高效液相色谱-二极管阵列检测器-四极杆飞行时间串联质谱仪(HSCCC-HSCCC)离线联用，系统分析了藤茶中的抗氧化剂。首先，采用1，1-二苯基-2-苦基肼自由基-高效液相色谱(DPPH-HPLC)快速筛选抗氧化剂。随后，以石油醚-乙酸乙酯-甲醇-水(4：9：4：9，v/v)和(4：9：5：8，v/v)为溶剂体系，对分步HSCCC法从藤茶乙酸乙酯提取物中分离和浓缩抗氧化剂进行了优化。最后，采用心脏切割法收集HSCCC的5个组分，进行高效液相-DAD-QTOE-MS/MS联用分析。HSCCC和HPLC之间良好的正交性导致了15个抗氧化性黄酮类化合物的鉴定，而4个次要的黄酮类化合物在藤茶中首次报道。结果表明，该系统能有效地从复杂的天然中草药中综合开发抗氧化剂。(C)2016爱思唯尔有限公司。保留所有权利。
Vine tea with strong antioxidant activity is commonly consumed as healthy tea/beverage. However, detailed information about its antioxidants is incomplete. Here, off-line hyphenation of heart-cutting high-speed countercurrent chromatography (HSCCC) with high performance liquid chromatography diode array detector-quadrupole time-of-flight tandem mass spectrometry (HPLC-DAD-QTOF-MS/MS) were described for systematic profiling antioxidants in vine tea. At first, antioxidants were rapidly screened by 1,1-diphenyl-2-picryl-hydrazyl radical-high performance liquid chromatography (DPPH-HPLC). Subsequently, stepwise HSCCC using petroleum ether-ethyl acetate-methanol-water (4:9:4:9, v/v/v/v) and (4:9:5:8, v/v/v/v) as solvent systems was optimized to fractionate and enrich antioxidants from ethyl acetate fraction of vine tea. Finally, heart-cutting mode was used to collect five interesting HSCCC fractions for HPLC-DAD-QTOE-MS/MS analysis. Desirable orthogonality between HSCCC and HPLC led to identification of fifteen antioxidant flavonoids, while four minor flavonoids were first reported in vine tea. Results showed that the developed system is efficient to comprehensively explore antioxidants from complex natural herbs. (C) 2016 Elsevier Ltd. All rights reserved.