Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture
Analyzing Structural Plasticity of Dendritic Spines in Organotypic Slice Culture
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DOI：
10.1007/978-1-4939-6688-2_19
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发表时间：
2017-01-01
期刊：
影响因子：
--
通讯作者：
Ribic, Adema
中科院分区：
文献类型：
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作者：
De Roo, Mathias;Ribic, Adema
了解发育、学习和神经系统疾病背景下突触动力学的规则是神经科学多个领域的重要组成部分。尽管方法论取得了重大进展，但突触结构动力学的观察仍然面临着重大的实验挑战。在本章中，我们描述了一套技术，可以在啮齿动物海马的器官型培养物中重复观察体外突触结构。我们描述了切片培养、用携带报告基因的质粒转染、用共焦激光扫描显微镜对树突棘进行重复成像以及树突棘形态动力学分析。
Understanding the rules of synapse dynamics in the context of development, learning, and nervous system disorders is an important part of several fields of neuroscience. Despite significant methodological advances, observations of structural dynamics of synapses still present a significant experimental challenge. In this chapter we describe a set of techniques that allow repetitive observations of synaptic structures in vitro in organotypic cultures of rodent hippocampus. We describe culturing of slices, transfection with reporter-carrying plasmids, repetitive imaging of dendritic spines with confocal laser scanning microscopy and analysis of spine morphology dynamics.