Implication of the RDRio Mycobacterium tuberculosis sublineage in multidrug resistant tuberculosis in Portugal
Implication of the RDRio Mycobacterium tuberculosis sublineage in multidrug resistant tuberculosis in Portugal
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DOI：
10.1016/j.meegid.2012.04.021
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发表时间：
2012-10-01
影响因子：
3.2
通讯作者：
de Sousa, Jose Germano
中科院分区：
文献类型：
--
作者：
David, Susana;Duarte, Elsa L.;de Sousa, Jose Germano
多药耐药和广泛耐药结核分枝杆菌是结核病控制计划的威胁。基因分型方法，如spoligotyping和MIRU-VNTR分型（分枝杆菌分散重复单位），是有用的监测潜在的流行菌株和估计菌株系统发育谱系和/或基因型家族。M.结核病拉丁美洲地中海（LAM）家族是结核病（TB）的主要全球贡献者。LAM特异性分子标记，Ag 85 C（103）单核苷酸多态性（SNP）和RDRio长序列多态性（LSP），用于表征来自葡萄牙的859例患者分离株的spoligotype签名。发现LAM菌株占所有结核病例的57.7%。RDRio缺失株（简称RDRio）占所有菌株的1/3，占多药耐药（MDR）菌株的60%以上。主要的spoligotype签名SIT 20属于LAM 1 RDRio亚系，占所有菌株的近1/5，其中超过20%是MDR。根据规定的12个位点MIRU-VNTR RDRio签名对已发表的数据集进行分析，发现96.3%（129/134）的MDR和广泛耐药（XDR）簇是RDRio。这是第一份将LAM RDRio亚系与MDR联系起来的报告。这些结果对监测这些传播增强的菌株，为今后努力遏制耐多药结核和广泛耐药结核做出了重要贡献。(c)2012 Elsevier B. V.保留所有权利。
Multidrug and extensively drug resistant Mycobacterium tuberculosis are a threat to tuberculosis control programs. Genotyping methods, such as spoligotyping and MIRU-VNTR typing (Mycobacterial Interspersed Repetitive Units), are useful in monitoring potentially epidemic strains and estimating strain phylogenetic lineages and/or genotypic families. M. tuberculosis Latin American Mediterranean (LAM) family is a major worldwide contributor to tuberculosis (TB). LAM specific molecular markers, Ag85C(103) single nucleotide polymorphism (SNP) and RDRio long-sequence polymorphism (LSP), were used to characterize spoligotype signatures from 859 patient isolates from Portugal. LAM strains were found responsible for 57.7% of all tuberculosis cases. Strains with the RDRio deletion (referred to as RDRio) were estimated to represent 1/3 of all the strains and over 60% of the multidrug resistant (MDR) strains. The major spoligotype signature SIT20 belonging to the LAM1 RDRio sublineage, represented close to 1/5th of all the strains, over 20% of which were MDR. Analysis of published datasets according to stipulated 12 loci MIRU-VNTR RDRio signatures revealed that 96.3% (129/134) of MDR and extensively drug resistant (XDR) clusters were RDRio. This is the first report associating the LAM RDRio sublineage with MDR. These results are an important contribution to the monitoring of these strains with heightened transmission for future endeavors to arrest MDR-TB and XDR-TB. (c) 2012 Elsevier B.V. All rights reserved.