An aptamer-based hook-effect-recognizable three-line lateral flow biosensor for rapid detection of thrombin
An aptamer-based hook-effect-recognizable three-line lateral flow biosensor for rapid detection of thrombin
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DOI：
10.1016/j.bios.2019.03.036
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发表时间：
2019-05-15
影响因子：
12.6
通讯作者：
Wang, Shengfu
中科院分区：
文献类型：
--
作者：
Gao, Ya;Zhu, Ziyu;Wang, Shengfu
本文在传统的两线LFB基础上加入凝血酶线，成功地建立了一种三线LFB，用于检测多种人血清中的凝血酶。我们在测试线和对照线之间引入凝血酶线。样品中凝血酶的浓度与LFB的三条线上的信号形成定量相关。通过数据处理，可以利用三条谱线上的信号对凝血酶进行定量测定。在10分钟内测量的凝血酶浓度的检测范围为1 nM至100 μ M，LOD为0.85 nM。我们的方法为快速和灵敏的凝血酶检测铺平了道路，并为在广泛的生理浓度范围内进行测试提供了上级设备，该设备也可以用于其他钩效应限制的适体或基于抗体的夹心LFB，并且即使在钩效应范围内也具有高准确度。
In this paper, a three-line LFB was successfully developed by adding a thrombin line to a conventional two-line LFB for the detection of thrombin in a wide range of human serum. We introduced a thrombin line between the test line and the control line. The concentration of thrombin in the sample was quantitatively related to the signal formation on the three lines of the LFB. We can make use of signal on three lines to quantitative determinate the thrombin by data processing. The detection range of thrombin concentrations measured in 10 min was 1 nM to 100 mu M and the LOD was 0.85 nM. Our approach paves way for rapid and sensitive thrombin detection and a superior device for testing in a wide range of physiological concentrations, which also can be used in other hook-effect-limited aptamers or antibodies based sandwich LFBs, and has a high accuracy even within the range of the hook-effect.