Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results.
Atomic force microscopy of bacteria from periodontal subgingival biofilm: Preliminary study results.
复制标题
DOI：
10.4103/1305-7456.110155
复制
发表时间：
2013-04
影响因子：
--
通讯作者：
Cicciù M
中科院分区：
文献类型：
--
作者：
Germano F;Bramanti E;Arcuri C;Cecchetti F;Cicciù M
原子力显微镜（AFM）是一种允许分析生物膜内细菌的纳米级形态的技术，并提供可能更好地用于理解细菌相互作用在牙周病中的作用的细节。选择5例牙周袋≥5 mm的泛发性牙周炎患者和5例轻度牙龈炎患者作为研究对象。收集细菌生物膜，并通过AFM应用进行形态学研究。这项研究揭示了牙周炎细菌是如何通过细胞壁的特定形态特征来表征的。通过三维重建显示细菌表面细节，再现了引起牙周病的主要代表性细菌种类。通过AFM分析，已经确定了复杂的糖萼结构、噬菌体样囊泡、螺旋体（经典和囊状形态）和细菌共聚集的存在。结果表明，原子力显微镜是一种可靠的技术，用于研究细菌的形态和检查微生物在牙菌斑中的相互作用。
Atomic force microscope (AFM) is a technology that allows analysis of the nanoscale morphology of bacteria within biofilm and provides details that may be better useful for understanding the role of bacterial interactions in the periodontal disease. Five patients with periodontal ≥5 mm pockets diagnosed as generalized periodontitis and five patients with slight gingivitis were selected for the investigation. Bacteria biofilms were collected and morphologically investigated by AFM application. The investigation revealed how periodontitis bacteria are characterized by specific morphologic features of the cell wall. The major representative species of bacteria causing periodontal diseases have been reproduced by a three-dimensional reconstruction showing the bacteria surface details. The presence of complex glycocalyx structures, bacteriophage-like vesicles, spirochetes (classic and cystic morphology) and bacterial co-aggregation has been identified by the AFM analysis. The results suggest that AFM is a reliable technique for studying bacterial morphology and for examining microbial interactions in dental plaque.