Promotion by burn stress of the translocation of bacteria from the gastrointestinal tracts of mice.
Promotion by burn stress of the translocation of bacteria from the gastrointestinal tracts of mice.
复制标题
烧伤应激促进小鼠胃肠道细菌的易位。
作者：
K. Maejima;E. Deitch;R. Berg
建立了小鼠烧伤模型，以测试非致死性热损伤对固有细菌从胃肠道（GI）转移到其他器官的影响。无特定病原体（SPF）小鼠给予15%或30%总体表面积烧伤，并在不同时间间隔对肠系膜淋巴结（MLN）、脾、肝、血液和腹腔进行易位细菌培养。在这些小鼠MLN的培养物中未生长出本土植物群的活需氧菌、兼性厌氧菌或严格厌氧菌。因此，SPF小鼠进行抗生素去污，然后用大肠杆菌定殖，以开发一种模型，其中大肠杆菌保持异常高的盲肠群体水平，并连续易位到MLN。这些小鼠在大肠杆菌定植后四天接受15%或30%的热烧伤。与对照组相比，随着烧伤面积的增加，细菌移位的发生率和大肠杆菌移位到MLN、脾、肝、血液和腹腔的数量增加。接受15%烧伤的小鼠不能从其血液、MLN或肝脏中清除静脉内攻击的大肠杆菌。因此，烧伤应激促进细菌从胃肠道转移到其他器官，引起菌血症。
A mouse burn model was established to test the effect of nonlethal thermal injury on the translocation of indigenous bacteria from the gastrointestinal (GI) tract to other organs. Specific pathogen-free (SPF) mice were given 15% or 30% total body surface area burns, and the mesenteric lymph nodes (MLNs), spleens, livers, blood, and peritoneal cavities were cultured for translocated bacteria at various time intervals. No viable aerobic, facultatively anaerobic, or strictly anaerobic bacteria of the indigenous flora grew in cultures from the MLNs of these mice. Consequently, SPF mice were antibiotic decontaminated and then colonized with Escherichia coli to develop a model in which E coli maintains abnormally high cecal population levels and translocates continuously to the MLN. These mice received 15% or 30% thermal burns four days after colonization with E coli. The incidence of bacterial translocation and the numbers of E coli translocating to the MLN, spleen, liver, blood, and peritoneal cavity increased with increasing burn area compared with controls. Mice receiving 15% burns could not clear intravenously challenged E coli from their bloodstream, MLN, or liver. Thus, burn stress promotes the translocation of bacteria from the GI tract to other organs to cause bacteremia.