Ocular allergy modulation to hi-dose antigen sensitization is a Treg-dependent process.
Ocular allergy modulation to hi-dose antigen sensitization is a Treg-dependent process.
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DOI：
10.1371/journal.pone.0075769
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Saban DR
中科院分区：
文献类型：
--
作者：
Lee HS;Schlereth S;Khandelwal P;Saban DR
A reproducible method to inhibit allergic immune responses is accomplished with hi-dose Ag sensitization, via intraperitoneal (IP) injection. However, the role of CD4+ CD25+ FoxP3+ T regulatory cells (Treg) in this process is unknown, as is whether such modulation extends to ocular allergy. We therefore determined herein whether hi-dose sensitization modulates ocular allergy, and whether CD4+ CD25+ FoxP3+ Treg are involved. C57BL/6 mice were IP sensitized via low-dose (100 µg) versus hi-dose (1000 µg) ovalbumin (OVA), in aluminum hydroxide (1 mg) and pertussis-toxin (300 ng). Other mice received anti-CD25 Ab (PC61) to ablate Treg during sensitization. In another experiment, Treg from hi-dose sensitized mice were adoptively transferred into low-dose sensitized mice. Once daily OVA challenges were administered. Clinical signs, IgE, T cell cytokines, and eosinophils were assessed. Data revealed that hi-dose, but not low-dose, sensitization led to allergy modulation, indicated by decreased clinical signs, serum IgE levels, Th2 recall responses, and eosinophil recruitment. T cells from hi-dose sensitized mice showed a robust increase in TGF-b production, and Treg from these mice were able to efficiently suppress effector T cell proliferation in vitro. In addition, in vivo Treg ablation in hi-dose sensitized mice revoked allergy modulation. Lastly, Treg from hi-dose sensitized mice were able to adoptively transfer allergy modulation to their low-dose sensitized counterparts. Collectively, these findings indicate that modulation to hi-dose sensitization, which is extended to ocular allergy, occurs in a Treg-dependent manner. In addition, our data suggest that hi-dose sensitization may henceforth facilitate the further examination of CD4+ CD25+ FoxP3+ Treg in allergic disease.
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DOI：
10.1084/jem.20051166
发表时间：
2005-12-05
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1164/rccm.200902-0190oc
发表时间：
2010-01-01
影响因子：
24.7
作者：
Aguilar-Pimentel, Juan A.;Alessandrini, Francesca;Ollert, Markus
通讯作者：
Ollert, Markus
影响因子：
6.1
作者：
Boudousquie, C.;Pellaton, C.;Spertini, F.
通讯作者：
Spertini, F.
影响因子：
14.2
作者：
Molet, S;Hamid, Q;Chakir, J
通讯作者：
Chakir, J
影响因子：
6.1
作者：
Barwig, C.;Raker, V.;Sudowe, S.
通讯作者：
Sudowe, S.