Complex genetic, photothermal, and photoacoustic analysis of nanoparticle-plant interactions
Complex genetic, photothermal, and photoacoustic analysis of nanoparticle-plant interactions
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DOI：
10.1073/pnas.1008856108
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发表时间：
2011-01-18
影响因子：
11.1
通讯作者：
Zharov, Vladimir P.
中科院分区：
文献类型：
--
作者：
Khodakovskaya, Mariya V.;de Silva, Kanishka;Zharov, Vladimir P.
了解工程纳米材料和植物之间的相互作用的性质是至关重要的理解纳米技术对环境和农业的影响，重点是毒性问题，植物疾病治疗和基因工程。迄今为止，在单个纳米颗粒和遗传水平上研究纳米颗粒与植物相互作用的进展甚微。在这里，我们介绍了一个先进的平台，集成遗传，拉曼，光热和光声方法。使用这种方法，我们发现多壁碳纳米管诱导番茄叶和根中基因表达的以前未知的变化，特别是与胁迫相关的基因的上调，包括病原体和水通道LeAqp 2基因诱导的基因。纳米气泡放大光热/光声成像，光谱和燃烧技术证明了根，叶和水果中的多壁碳纳米管的检测到单个纳米颗粒和细胞水平。因此，与现有的检测方法相比，我们的集成平台可以以更高的灵敏度和特异性研究纳米颗粒对植物的影响。
Understanding the nature of interactions between engineered nanomaterials and plants is crucial in comprehending the impact of nanotechnology on the environment and agriculture with a focus on toxicity concerns, plant disease treatment, and genetic engineering. To date, little progress has been made in studying nanoparticle-plant interactions at single nanoparticle and genetic levels. Here, we introduce an advanced platform integrating genetic, Raman, photothermal, and photoacoustic methods. Using this approach, we discovered that multiwall carbon nanotubes induce previously unknown changes in gene expression in tomato leaves and roots, particularly, up-regulation of the stress-related genes, including those induced by pathogens and the water-channel LeAqp2 gene. A nano-bubble amplified photothermal/photoacoustic imaging, spectroscopy, and burning technique demonstrated the detection of multiwall carbon nanotubes in roots, leaves, and fruits down to the single nanoparticle and cell level. Thus, our integrated platform allows the study of nanoparticles' impact on plants with higher sensitivity and specificity, compared to existing assays.